Preliminary Insights into the Phylogeography of Six Aquatic Hyphomycete Species 英文参考文献.docVIP
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Preliminary Insights into the Phylogeography of Six Aquatic Hyphomycete Species 英文参考文献
PreliminaryInsightsintothePhylogeographyofSix
AquaticHyphomyceteSpecies
SofiaDuarte1*,SahadevanSeena1,FelixBa¨rlocher2,FernandaCa′ssio1,Cla′udiaPascoal1
1CentreofMolecularandEnvironmentalBiology (CBMA),DepartmentofBiology,UniversityofMinho, CampusdeGualtar, Braga,Portugal,2DepartmentofBiology,
MountAllisonUniversity,Sackville,NewBrunswick,Canada
Abstract
Aquatichyphomycetesoccurworldwideonawiderangeofplantsubstratesdecomposinginfreshwaters,andareknownto
playakeyroleinorganicmatterturnover.Thepresumedworldwidedistributionofmanyaquatichyphomycetespecieshas
beenbasedonmorphology-basedtaxonomyandidentification,whichmayoverlookcrypticspecies,andmaskglobal-scale
biogeographicalpatterns.ThismightbecircumventedbyusingDNAsequencedata.Theinternaltranscribedspacer(ITS)
region from rDNA was recently designated as the most suitable barcode for fungal identification. In this study, we
generatedITSbarcodesof130isolatesbelongingto6aquatichyphomycetespecies(Anguillosporafiliformis,Flagellospora
penicillioides,Geniculosporagrandis,Lunulosporacurvula,TetrachaetumelegansandTricladiumchaetocladium),andcollected
fromstreamsofSouthwestEurope(86isolates)andEastAustralia(44isolates).EuropeanandAustralianpopulationsof4
species(A.filiformis,F.penicillioides,G.grandisandT.elegans)groupedintodifferentclades,andmoleculardiversityindices
supportedsignificantdifferentiation.Continentsdidnotsharehaplotypes,exceptforT.chaetocladium.Overallresultsshow
substantialpopulationdiversityforalltestedspeciesandsuggeststhatthebiogeographyofaquatichyphomycetesmaybe
species-specific.
Citation: Duarte S, Seena S, Ba¨rlocher F, Ca′ssio F, Pascoal C (2012) Preliminary Insights into the Phylogeographyof Six Aquatic Hyphomycete Species. PLoS
ONE7(9):e45289.doi:10.1371/journal.pone.0045289
Editor:JasonE.Stajich,UniversityofCaliforniaRiverside,UnitedStatesofAmerica
ReceivedJune4,2012;AcceptedAugust14,2012;PublishedSeptember18,2012
Copyright: ? 2012 Duarte et al. This is an open-access article distributed unde
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