Principles of Carbon Catabolite Repression in the Rice Blast Fungus Tps1, Nmr1-3, and a MATE–Family Pump Regulate Glucose Metabolism during Infection 英文参考文献.docVIP

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Principles of Carbon Catabolite Repression in the Rice Blast Fungus Tps1, Nmr1-3, and a MATE–Family Pump Regulate Glucose Metabolism during Infection 英文参考文献.doc

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Principles of Carbon Catabolite Repression in the Rice Blast Fungus Tps1, Nmr1-3, and a MATE–Family Pump Regulate Glucose Metabolism during Infection 英文参考文献

PrinciplesofCarbonCataboliteRepressionintheRice BlastFungus:Tps1,Nmr1-3,andaMATE–FamilyPump RegulateGlucoseMetabolismduringInfection JessieFernandez1.,JanetD.Wright1.,DavidHartline1,CristianF.Quispe1, NandakumarMadayiputhiya2,RichardA.Wilson1* 1DepartmentofPlantPathology,UniversityofNebraska–Lincoln,Lincoln,Nebraska,UnitedStatesofAmerica,2ProteomicandMetabolomicCoreFacility,RedoxBiology Center,DepartmentofBiochemistry,UniversityofNebraska–Lincoln,Lincoln,Nebraska,UnitedStatesofAmerica Abstract Understandingthegeneticpathwaysthatregulatehowpathogenicfungirespondtotheirenvironmentisparamountto developing effective mitigation strategies against disease. Carbon catabolite repression (CCR) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourablecarbonsources,butlittleisknownaboutthecomponentsofCCRinfilamentousfungi.Herewereportthreenew mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae: the sugar sensor Tps1, the Nmr1-3 inhibitor proteins, and the multidrug and toxin extrusion (MATE)–family pump, Mdt1. Using simple plate tests coupled with transcriptionalanalysis,weshowthatTps1,inresponsetoglucose-6-phosphatesensing,triggersCCRviatheinactivationof Nmr1-3.Inaddition,bydissectingtheCCRpathwayusingAgrobacteriumtumefaciens-mediatedmutagenesis,wealsoshow thatMdt1isanadditionalandpreviouslyunknownregulatorofglucosemetabolism.Mdt1regulatesglucoseassimilation downstream of Tps1 and is necessary for nutrient utilization, sporulation, and pathogenicity. This is the first functional characterization of a MATE–family protein in filamentous fungi and the first description of a MATE protein in genetic regulationorplantpathogenicity.PerturbingCCRinDtps1andMDT1disruptionstrainsthusresultsinphysiologicaldefects that impact pathogenesis, possibly through the early expression of cell wall–degrading enzymes. Taken together, the importance of discovering three new regulators