Probiotic Encapsulation Technology From Microencapsulation to Release into the Gut 英文参考文献.docVIP

Pharmaceutics 2012, 4, 149-163; doi:10.3390/pharmaceutics4010149 OPEN ACCESS Pharmaceutics ISSN 1999-4923 /journal/pharmaceutics Article Probiotic Encapsulation Technology: From Microencapsulation to Release into the Gut Gildas K. Gbassi 1,2 and Thierry Vandamme 1,* 1 Laboratoire de Conception et Application de Molécules Bioactives (UMR-7199), Faculté de Pharmacie, UdS-CNRS, 74 Route du Rhin, 67401 Illkirch-Graffenstaden, France 2 Département de Chimie Générale et Minérale, Faculté de Pharmacie, Université de Cocody, 01 BPV 34, Abidjan, Cote d’Ivoire * Author to whom correspondence should be addressed; E-Mail: vandamme@unistra.fr; Tel.: +33-3-68-85-41-06; Fax: +33-3-68-85-43-25. Received: 21 December 2011; in revised form: 20 January 2012 / Accepted: 31 January 2012 / Published: 6 February 2012 Abstract: Probiotic encapsulation technology (PET) has the potential to protect microorgansisms and to deliver them into the gut. Because of the promising preclinical and clinical results, probiotics have been incorporated into a range of products. However, there are still many challenges to overcome with respect to the microencapsulation process and the conditions prevailing in the gut. This paper reviews the methodological approach of probiotics encapsulation including biomaterials selection, choice of appropriate technology, in vitro release studies of encapsulated probiotics, and highlights the challenges to be overcome in this area. Keywords: biomaterials; microencapsulation; probiotics; protective device; artificial media; cells release Abbreviations PET, probiotic encapsulation technology; M, mannuronic acid; G, guluronic acid; FDA, food and drug administration; FAO, food and agricultural organization; WHO, world health organization; CAP, cellulose acetate phthalate; ASM, american society of microbiology; SDS, sodium dodecyl sulphate polyacrylamide gel electrophoresis; FTIR-ATR, fo