Production of Hesperetin Glycosides by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activities 英文参考文献.docVIP

Nutrients 2010, 2, 171-180; doi:10.3390/nu2020171 OPEN ACCESS nutrients ISSN 2072-6643 /journal/nutrients Article Production of Hesperetin Glycosides by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activities Kei Shimoda 1,* and Hiroki Hamada 2,* 1 Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan 2 Department of Life Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama 700-0005, Japan * Author to whom correspondence should be addressed: E-Mail: shimoda@med.oita-u.ac.jp (K.S.); Tel.: +81-97-586-5606; Fax: +81-97-586-5619 (K.S.); E-Mail: hamada@dls.ous.ac.jp (H.H.); Tel.: +81-86-256-9473; Fax: +81-86-256-8468 (H.H.) Received: 7 January 2010 / Accepted: 1 February 2010 / Published: 9 February 2010 Abstract: The production of hesperetin glycosides was investigated using glycosylation with Xanthomonas campestris and cyclodextrin glucanotransferase (CGTase). X. campestris glucosylated hesperetin to its 3-, 5-, and 7-O-glucosides, and CGTase converted hesperetin glucosides into the corresponding maltosides. The resulting 7-O- glucoside and 7-O-maltoside of hesperetin showed inhibitory effects on IgE antibody production and on O2- generation from rat neutrophils. Keywords: Hesperetin; Xanthomonas campestris; cyclodextrin glucanotransferase; glycoside; anti-allergic activity 1. Introduction Vitamin P is the term used for bioactive flavonoids. Hesperetin (5,7,3-trihydroxyl-4-methoxyl- flavanone), which occurs in Citrus species, tomatoes, and apples, is a kind of vitamin P and has been well documented for its medicinal properties as an important Chinese traditional medicine [1,2]. Hesperetin has been studied for its effects on the blood-brain barrier, signal transduction pathway, and  Nutrients 2010, 2 172 certain kinds of cancer [3-6]. However, hesperetin barely d