Proteomic patterns analysis with multivariate calculations as a promising tool for prompt differentiation of early stage lung tissue with cancer and unchanged tissue material 英文参考文献.docVIP

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Proteomic patterns analysis with multivariate calculations as a promising tool for prompt differentiation of early stage lung tissue with cancer and unchanged tissue material 英文参考文献.doc

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Proteomic patterns analysis with multivariate calculations as a promising tool for prompt differentiation of early stage lung tissue with cancer and unchanged tissue material 英文参考文献

Waloszczyketal.DiagnosticPathology2011,6:22 /content/6/1/22 RESEARCH OpenAccess Proteomicpatternsanalysiswithmultivariate calculationsasapromisingtoolforprompt differentiationofearlystagelungtissuewith cancerandunchangedtissuematerial PiotrWaloszczyk1,TomaszJanus1*,JacekAlchimowicz2,TomaszGrodzki2,KrzysztofBorowiak1 Abstract Background:Lungcancerdiagnosisintissuematerialwithcommonlyusedhistologicaltechniquesissometimes inconvenientandinanumberofcasesleadstoambiguousconclusions.Frequentlyadvancedimmunostaining techniqueshavetobeemployed,yettheyarebothtimeconsumingandlimited.Inthisstudyaproteomic approachispresentedwhichmayhelpprovideunambiguouspathologicdiagnosisoftissuematerial. Methods:Lungtissuematerialfoundtobepathologicallychangedwaspreparedtoisolateproteomewithfast andnonselectiveprocedure.Isolatedpeptidesandproteinsinrangingfrom3.5to20kDawereanalyseddirectly usinghighresolutionmassspectrometer(MALDI-TOF/TOF)withsinapicacidasamatrix.Recordedcomplexspectra ofasinglerunwerethenanalyzedwithmultivariatestatisticalanalysisalgorithms(principlecomponentanalysis, classificationmethods).Intheappliedprotocolwefocusedonobtainingthespectrarichestinproteinsignals constitutingapatternofchangewithinthesamplecontainingdetailedinformationaboutitsproteincomposition. Advancedstatisticalmethodsweretoindicatedifferencesbetweenexaminedgroups. Results:Obtainedresultsindicatechangesinproteomeprofilesofchangedtissuesincomparisonto physiologicallyunchangedmaterial(controlgroup)whichwerereflectedintheresultofprinciplecomponent analysis(PCA).Pointsrepresentingspectraofcontrolgroupwerelocatedindifferentareasofmultidimensional spaceandwerelessdiffusedincomparisontocancertissues.Threedifferentclassificationalgorithmsshowed recognitioncapabilityof100%regardingclassificationofexaminedmaterialintoanappropriategroup. Conclusion:Theapplicationofthepresentedprotocolandmethodenabledfindingpathologicalchangesintissue materialregardlessoflocalizationandsizeofabnormalitiesinthesamplevolume.Proteomic