Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases 英文参考文献.docVIP

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Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases 英文参考文献.doc

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Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases 英文参考文献

ProteomicInterrogationofAndrogenActioninProstate CancerCellsRevealsRolesofAminoacyltRNA Synthetases AdaikkalamVellaichamy1,2,ArunSreekumar1,2,6¤*,JohnR.Strahler5,TheckelnayckeRajendiran1,2, JindanYu1,2,7,SooryanarayanaVarambally1,2,6,YongLi1,2,GilbertS.Omenn3,4,6,ArulM. Chinnaiyan1,2,6.,AlexeyI.Nesvizhskii2,3. * 1MichiganCenterforTranslationalPathology,UniversityofMichigan,AnnArbor,Michigan,UnitedStatesofAmerica,2DepartmentofPathology,UniversityofMichigan, AnnArbor,Michigan,UnitedStatesofAmerica,3CenterforComputationalMedicineandBioinformatics,UniversityofMichigan,AnnArbor,Michigan,UnitedStatesof America,4InternalMedicineandHumanGenetics,UniversityofMichigan,AnnArbor,Michigan,UnitedStatesofAmerica,5MichiganProteomeConsortium,Universityof Michigan, Ann Arbor, Michigan, United States of America, 6Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan, United States of America, 7NorthwesternUniversityFeinbergSchoolofMedicine,Chicago,Illinois,UnitedStatesofAmerica Abstract Prostate cancer remains the most common malignancy among men in United States, and there is no remedy currently available for the advanced stage hormone-refractory cancer. This is partly due to the incomplete understanding of androgen-regulated proteins and their encoded functions. Whole-cell proteomes of androgen-starved and androgen- treatedLNCaPcellswereanalyzedbysemi-quantitativeMudPITESI-iontrapMS/MSandquantitativeiTRAQMALDI-TOF MS/MSplatforms,withidentificationofmorethan1300high-confidenceproteins.Anenrichment-basedpathwaymapping of the androgen-regulated proteomic data sets revealed a significant dysregulation of aminoacyl tRNA synthetases, indicatinganincreaseinproteinbiosynthesis-ahallmarkduringprostatecancerprogression.Thisobservationissupported by immunoblot and transcript data from LNCaP cells, and prostate cancer tissue. Thus, data derived from multiple proteomicsplatformsandtranscriptdatacoupledwithinformaticsanalysisprovidesadeeperinsightintothefunctional consequenceso