Proteomic Profiling of Human Keratinocytes Undergoing UVB-Induced Alternative Differentiation Reveals TRIpartite Motif Protein 29 as a Survival Factor 英文参考文献.docVIP
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Proteomic Profiling of Human Keratinocytes Undergoing UVB-Induced Alternative Differentiation Reveals TRIpartite Motif Protein 29 as a Survival Factor 英文参考文献
ProteomicProfilingofHumanKeratinocytesUndergoing
UVB-InducedAlternativeDifferentiationReveals
TRIpartiteMotifProtein29asaSurvivalFactor
Ve′roniqueBertrand-Vallery1,NathalieBelot2,MarcDieu1,EdouardDelaive1,Noe¨lleNinane1 ,Catherine
Demazy1,MartineRaes1,MichelSalmon2,YvesPoumay3,FlorenceDebacq-Chainiaux1 ,Olivier
Toussaint1*
1ResearchUnitofCellularBiology,UniversityofNamur(FUNDP),Namur,Belgium,2StratiCELLSA,Isnes,Belgium,3CellandTissueLaboratory,URPHYM,Universityof
Namur(FUNDP),Namur,Belgium
Abstract
Background: Repeated exposures to UVB of human keratinocytes lacking functional p16INK-4a and able to differentiate
induceanalternativestateofdifferentiationratherthanstress-inducedprematuresenescence.
Methodology/PrincipalFindings:A2D-DIGEproteomicprofilingofthisalternativestateofdifferentiationwasperformed
herein at various times after the exposures to UVB. Sixty-nine differentially abundant protein species were identified by
massspectrometry,manyofwhichareinvolvedinkeratinocytedifferentiationandsurvival.Amongtheseproteinspecies
was TRIpartite Motif Protein 29 (TRIM29). Increased abundance of TRIM29 following UVB exposures was validated by
WesternblotusingspecificantibodyandwasalsofurtheranalysedbyimmunochemistryandbyRT-PCR.TRIM29wasfound
very abundant in keratinocytes and reconstructed epidermis. Knocking down the expression of TRIM29 by short-hairpin
RNAinterferencedecreasedtheviabilityofkeratinocytesafterUVBexposure.TheabundanceofinvolucrinmRNA,amarker
oflatedifferentiation,increasedconcomitantly.InTRIM29-knockeddownreconstructedepidermis,thepresenceofpicnotic
cellsrevealedcellinjury.IncreasedabundanceofTRIM29wasalsoobserveduponexposuretoDNAdamagingagentsand
PKCactivation.TheUVB-inducedincreaseofTRIM29abundancewasdependentonaPKCsignalingpathway,likelyPKCd.
Conclusions/Significance: These findings suggest that TRIM29 allows keratinocytes to enter a protective alternative
differentiationprocessratherthandiemassivelyafterstress.
Citation:Bertrand-ValleryV,BelotN,DieuM
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