Proteomics of Protein Secretion by Aggregatibacter actinomycetemcomitans 英文参考文献.docVIP

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Proteomics of Protein Secretion by Aggregatibacter actinomycetemcomitans 英文参考文献.doc

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Proteomics of Protein Secretion by Aggregatibacter actinomycetemcomitans 英文参考文献

ProteomicsofProteinSecretionbyAggregatibacter actinomycetemcomitans VincentZijnge1,ThomasKieselbach2,JanOscarsson1* 1OralMicrobiology,DepartmentofOdontology,Umea? University,Umea?, Sweden,2DepartmentofChemistry,Umea? University,Umea?, Sweden Abstract The extracellular proteome (secretome) of periodontitis-associated bacteria may constitute a major link between periodontitisandsystemicdiseases.ToobtainanoverviewofthevirulencepotentialofAggregatibacteractinomycetemco- mitans,anoralandsystemichumanpathogenimplicatedinaggressiveperiodontitis,weusedacombinedLC-MS/MSand bioinformaticsapproachtocharacterizethesecretomeandproteinsecretionpathwaysoftherough-colonyserotypeastrain D7S. LC-MS/MS revealed 179 proteins secreted during biofilm growth. Further to confirming the release of established virulencefactors(e.g.cytolethaldistendingtoxin[CDT],andleukotoxin[LtxA]),weidentifiedadditionalputativevirulence determinantsinthesecretome.TheseincludedDegQ,fHbp,LppC,Macrophageinfectivityprotein(MIP),NlpB,Pcp,PotD, TolB, and TolC. This finding indicates that the number of extracellular virulence-related proteins is much larger than previouslydemonstrated,whichwasalsosupportedbyinsilicoanalysisofthestrainD7Sgenome.Moreover,ourLC-MS/MS and insilico data revealed that at least Type I, II, and V secretion are actively used to excrete proteins directly into the extracellularspace,orviatwo-steppathwaysinvolvingtheSec/Tatsystemsfortransportacrosstheinnermembrane,and outer membrane factors, secretins and auto-transporters, respectively for delivery across the outer membrane. Taken together,ourresultsprovideamolecularbasisforfurtherelucidatingtheroleofA.actinomycetemcomitansinperiodontal andsystemicdiseases. Citation: Zijnge V, Kieselbach T, Oscarsson J (2012) Proteomics of Protein Secretion by Aggregatibacter actinomycetemcomitans. PLoS ONE 7(7): e41662. doi:10.1371/journal.pone.0041662 Editor:JamunaraniVadivelu,UniversityofMalaya,Malaysia ReceivedApril25,2012;AcceptedJune25,2012;Pub