Quantitative 3-Dimensional Imaging of Murine Neointimal and Atherosclerotic Lesions by Optical Projection Tomography 英文参考文献.docVIP

Quantitative3-DimensionalImagingofMurine NeointimalandAtheroscleroticLesionsbyOptical ProjectionTomography NicholasS.Kirkby,LucindaLow,JonathanR.Seckl,BrianR.Walker,DavidJ.Webb,PatrickW.F. Hadoke* CentreforCardiovascularScience,UniversityofEdinburgh,Edinburgh,UnitedKingdom Abstract Objective: Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study to ‘snapshots’ within each vessel. This study was undertaken to determine the suitability of optical projection tomographic(OPT)imagingforthe3-dimensionalrepresentationandquantificationofintimallesionsinmousearteries. Methods and Results: Vascular injury was induced by wire-insertion or ligation of the mouse femoral artery or administration of an atherogenic diet to apoE-deficient mice. Lesion formation was examined by OPT imaging of autofluorescent emission. Lesions could be clearly identified and distinguished from the underlying vascular wall. Planimetricmeasurementsoflesionareacorrelatedwellwiththosemadefromhistologicalsectionssubsequentlyproduced from the same vessels (wire-injury: R2=0.92; ligation-injury: R2=0.89; atherosclerosis: R2=0.85), confirming both the accuracyofthismethodologyanditsnon-destructivenature.Itwasalsopossibletorecordvolumetricmeasurementsof lesionandlumenandthesewerehighlyreproduciblebetweenscans(coefficientofvariation=5.36%,11.39%and4.79%for wire-andligation-injuryandatherosclerosis,respectively). Conclusions:ThesedatademonstratetheeminentsuitabilityofOPTforimagingofatheroscleroticandneointimallesion formation,providingamuchneededmeansfortheroutine3-dimensionalanalysisofvascularmorphologyinstudiesofthis type. Citation:KirkbyNS,LowL,SecklJR,WalkerBR,WebbDJ,etal.(2011)Quantitative3-DimensionalImagingofMurineNeointimalandAtheroscleroticLesionsby OpticalProjectionTomography.PLoSONE6(2):e16906.doi:10.1371/journal.pone.0016906 Editor:AlmaZernecke,Universita¨t Wu¨rzburg,Germany ReceivedOctober6,2010;AcceptedJanuary17,2011;PublishedFebruary