Quantitative image analysis of immunohistochemical stains using a CMYK color model 英文参考文献.docVIP

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Quantitative image analysis of immunohistochemical stains using a CMYK color model 英文参考文献.doc

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Quantitative image analysis of immunohistochemical stains using a CMYK color model 英文参考文献

Diagnostic Pathology BioMedCentral Methodology Open Access Quantitative image analysis of immunohistochemical stains using a CMYK color model Nhu-An Pham1,2, Andrew Morrison1, Joerg Schwock1,3, Sarit Aviel-Ronen1, Vladimir Iakovlev1,3, Ming-Sound Tsao1,2,3, James Ho1 and David W Hedley*1,2,3 Address: 1Division of Applied Molecular Oncology, Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Toronto, Canada, 2Department of Medical Biophysics, University of Toronto, Toronto, Canada and 3Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada Email: Nhu-An Pham - npham@uhnres.utoronto.ca; Andrew Morrison - amorriso@uhnres.utoronto.ca; Joerg Schwock - jschwock@uhnres.utoronto.ca; Sarit Aviel-Ronen - saronen@uhnres.utoronto.ca; Vladimir Iakovlev - viakovle@uhnres.utoronto.ca; Ming-Sound Tsao - Ming.Tsao@uhn.on.ca; James Ho - james.ho@uhn.on.ca; David W Hedley* - dhedley@uhnres.utoronto.ca * Corresponding author Published: 27 February 2007 Received: 1 February 2007 Accepted: 27 February 2007 Diagnostic Pathology 2007, 2:8 doi:10.1186/1746-1596-2-8 This article is available from: /content/2/1/8 ? 2007 Pham et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Computer image analysis techniques have decreased effects of observer biases, and increased the sensitivity and the throughput of immunohistochemistry (IHC) as a tissue-based procedure for the evaluation of diseases. Methods: We adapted a Cyan/Magenta/Yellow/Key (CMYK) model for automated computer image analysis to quantify IHC stains in hematoxylin counterstained histological sections. Results: The spectral characteristics of the chromogens AEC, DAB and NovaRed as well as