Quantitative analysis of the epithelial lining architecture in radicular cysts and odontogenic keratocysts 英文参考文献.docVIP

Head Face Medicine BioMedCentral Research Open Access Quantitative analysis of the epithelial lining architecture in radicular cysts and odontogenic keratocysts Gabriel Landini* Address: Oral Pathology Unit. School of Dentistry, The University of Birmingham, St. Chads Queensway, Birmingham B4 6NN, UK Email: Gabriel Landini* - G.Landini@bham.ac.uk * Corresponding author Published: 17 February 2006 Received: 01 November 2005 Accepted: 17 February 2006 Head Face Medicine 2006, 2:4 doi:10.1186/1746-160X-2-4 This article is available from: /content/2/1/4 ? 2006 Landini; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: This paper describes a quantitative analysis of the cyst lining architecture in radicular cysts (of inflammatory aetiology) and odontogenic keratocysts (thought to be developmental or neoplastic) including its 2 counterparts: solitary and associated with the Basal Cell Naevus Syndrome (BCNS). Methods: Epithelial linings from 150 images (from 9 radicular cysts, 13 solitary keratocysts and 8 BCNS keratocysts) were segmented into theoretical cells using a semi-automated partition based on the intensity of the haematoxylin stain which defined exclusive areas relative to each detected nucleus. Various morphometrical parameters were extracted from these cells and epithelial layer membership was computed using a systematic clustering routine. Results: Statistically significant differences were observed across the 3 cyst types both at the morphological and architectural levels of the lining. Case-wise discrimination between radicular cysts and keratocyst was highly accurate (with an error of just 3.3%). However, the odontogenic keratocyst subtypes c