Rapid Detection and Differentiation of Swine-Origin Influenza A Virus (H1N12009) from Other Seasonal Influenza A Viruses 英文参考文献.docVIP

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Rapid Detection and Differentiation of Swine-Origin Influenza A Virus (H1N12009) from Other Seasonal Influenza A Viruses 英文参考文献.doc

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Rapid Detection and Differentiation of Swine-Origin Influenza A Virus (H1N12009) from Other Seasonal Influenza A Viruses 英文参考文献

Viruses 2012, 4, 3012-3019; doi:10.3390/v4113012 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article Rapid Detection and Differentiation of Swine-Origin Influenza A Virus (H1N1/2009) from Other Seasonal Influenza A Viruses Jiangqin Zhao 1,*, Xue Wang 1, Viswanath Ragupathy 1, Panhe Zhang 1, Wei Tang 1, Zhiping Ye 2, Maryna Eichelberger 2 and Indira Hewlett 1,* 1 Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA; E-Mails: xue.wang@ (X.W.); viswanath.ragupathy@ (V.R.); panhe.zhang@ (P.Z.); annietang_93@ (W.T.) 2 Division of Viral Products, Office of Vaccines Research and Review, CBER, Food and Drug Administration, Bethesda, MD 20892, USA; E-Mails: zhiping.ye@ (Z.Y.); maryna.eichelberger@ (M.E.) * Authors to whom correspondence should be addressed; E-Mails: jiangqin.zhao@ (J.Z.); indira.hewlett@ (I.H.); Tel.: +1-301-827-0795; Fax: +1-301-480-7928. Received: 25 August 2012; in revised form: 1 November 2012 / Accepted: 1 November 2012 / Published: 9 November 2012 Abstract: We previously developed a rapid and simple gold nanoparticle(NP)-based genomic microarray assay for identification of the avian H5N1 virus and its discrimination from other influenza A virus strains (H1N1, H3N2). In this study, we expanded the platform to detect the 2009 swine-origin influenza A virus (H1N1/2009). Multiple specific capture and intermediate oligonucleotides were designed for the matrix (M), hemagglutinin (HA), and neuraminidase (NA) genes of the H1N1/2009 virus. The H1N1/2009 microarrays were printed in the same format as those of the seasonal influenza H1N1 and H3N2 for the HA, NA, and M genes. Viral RNA was tested using capture-target-intermediate oligonucleotide hybridization and gold NP-mediated silver staining. The signal from the 4 capture-target-intermediates of the HA and NA genes was specific for H1N1/2009 virus and