Reactive Oxygen Species Scavenging Activity of Flavone Glycosides from Melilotus neapolitana 英文参考文献.docVIP

Molecules 2007, 12, 263-270 molecules ISSN 1420-3049 Full Paper Reactive Oxygen Species Scavenging Activity of Flavone Glycosides from Melilotus neapolitana Antonio Fiorentino*, Brigida D’Abrosca, Severina Pacifico, Annunziata Golino, Claudio Mastellone, Palma Oriano, and Pietro Monaco Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli – via Vivaldi 43, I-81100 Caserta, Italy * Author to whom correspondence should be addressed. E-mail: antonio.fiorentino@unina2.it Received: 21 January 2007; in revised form: 13 February 2007 / Accepted: 27 February 2007 / Published: 28 February 2007 Abstract: One new and six known flavone glycosides were isolated from the MeOH extract of Melilotus neapolitana Ten. The new compound, identified as 7-O-β-D-gluco- pyranosyloxy-4,5-dihydroxy-3-[O-α-L-rhamnopyranosyl-(1→6)-3-O-β-D-glucopyrano- syloxy]flavone (1) by 1D and 2D NMR techniques and mass spectra, was isolated along with kaempferol-3-O-rutinoside (2), kaempferol-3-O-glucoside (3), rutin (4), quercetin-3- O-glucoside (5), isorhamnetin-3-O-rutinoside (6), and isorhamnetin-3-O-glucoside (7). The antioxidant and radical scavenging activities of these compounds and the whole crude methanol extract were evaluated. The organic extract can inhibit MDA marker’s synthesis by 57%. All the metabolites displayed good reducing power, with the kaempferol (2,3) and isorhamnetin derivatives (6,7) being less active than the corresponding quercetin derivatives 4,5. Keywords: Melilotus neapolitana Ten., flavone glycosides, ROSs, antioxidant activity. Introduction Flavonoids are ubiquitous polyphenolic metabolites in plants that have diverse beneficial biochemical and antioxidant effects [1]. Their dietary intake is quite high, compared to other dietary antioxidants like vitamins C and E. The flavonoids have aroused considerable interest recently because of their potential beneficial ef