Real-Time Sensing of Cell Morphology by Infrared Waveguide Spectroscopy 英文参考文献.docVIP

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Real-Time Sensing of Cell Morphology by Infrared Waveguide Spectroscopy 英文参考文献.doc

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Real-Time Sensing of Cell Morphology by Infrared Waveguide Spectroscopy 英文参考文献

Real-TimeSensingofCellMorphologybyInfrared WaveguideSpectroscopy VictorYashunsky1*.,TalMarciano1.,VladislavLirtsman1,MichaelGolosovsky1,DanDavidov1, BenjaminAroeti2 1The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel, 2Department of Cell and Developmental Biology, The Alexander Silberman InstituteofLifeScience,TheHebrewUniversityofJerusalem,Jerusalem,Israel Abstract Wedemonstratethataliveepithelialcellmonolayercanactasaplanarwaveguide.Ourinfraredreflectivitymeasurements show that highly differentiated simple epithelial cells, which maintain tight intercellular connectivity, support efficient waveguidingoftheinfraredlightinthespectralregionof1.4–2.5 mmand3.5–4mm.Thewavelengthandthemagnitudeof the waveguide mode resonances disclose quantitative dynamic information on cell height and cell-cell connectivity. To demonstratethisweshowtwoexperiments.Inthefirstonewetraceinreal-timethekineticsofthedisruptionofcell-cell contacts induced by calcium depletion. In the second one we show that cell treatment with the PI3-kinase inhibitor LY294002resultsinaprogressivedecreaseincellheightwithoutaffectingintercellularconnectivity.Ourdatasuggestthat infraredwaveguidespectroscopycanbeusedasanovelbio-sensingapproachforstudyingthemorphologyofepithelial cellsheetsinreal-time,label-freemannerandwithhighspatial-temporalresolution. Citation: Yashunsky V, Marciano T, Lirtsman V, Golosovsky M, Davidov D, et al. (2012) Real-Time Sensing of Cell Morphology by Infrared Waveguide Spectroscopy.PLoSONE7(10):e48454.doi:10.1371/journal.pone.0048454 Editor:EshelBen-Jacob,TelAvivUniversity,Israel ReceivedJuly16,2012;AcceptedSeptember26,2012;PublishedOctober31,2012 Copyright: ?2012 Yashunsky etal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbytheNOFARgrantoftheIs