Regulating Repression Roles for the Sir4 N-Terminus in Linker DNA Protection and Stabilization of Epigenetic States 英文参考文献.docVIP

下载本文档

4
0
约9.43万字
约 19页
2017-05-13 发布于上海
举报
版权申诉

Regulating Repression Roles for the Sir4 N-Terminus in Linker DNA Protection and Stabilization of Epigenetic States 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Regulating Repression Roles for the Sir4 N-Terminus in Linker DNA Protection and Stabilization of Epigenetic States 英文参考文献

Regulating Repression: Roles for the Sir4 N-Terminus in Linker DNA Protection and Stabilization of Epigenetic States StephanieKueng1,MonikaTsai-Pflugfelder1,MarianoOppikofer1,2,HelderC.Ferreira1,EmmaRoberts1¤, ChinyenTsai1,Tim-ChristophRoloff1,RagnaSack1,SusanM.Gasser1,2* 1FriedrichMiescherInstituteforBiomedicalResearch,Basel,Switzerland,2FacultyofNaturalSciences,UniversityofBasel,Basel,Switzerland Abstract Silent information regulator proteins Sir2, Sir3, and Sir4 form a heterotrimeric complex that represses transcription at subtelomericregionsandhomothallicmatingtype(HM)lociinbuddingyeast.Wehaveperformedadetailedbiochemical andgeneticanalysisofthelargestSirprotein,Sir4.TheN-terminalhalfofSir4isdispensableforSIR–mediatedrepressionof HM loci in vivo, except in strains that lack Yku70 or haveweak silencer elements. For HM silencing in these cells, the C- terminal domain (Sir4C, residues 747–1,358) must be complemented with an N-terminal domain (Sir4N; residues 1–270), expressedeitherindependentlyorasafusionwithSir4C.Nonetheless,recombinantSir4CcanformacomplexwithSir2and Sir3 in vitro, is catalytically active, and has sedimentation properties similar to a full-length Sir4-containing SIR complex. Sir4C-containing SIR complexes bind nucleosomal arrays and protect linker DNA from nucleolytic digestion, but less effectively than wild-type SIR complexes. Consistently, full-length Sir4 is required for the complete repression of subtelomeric genes. Supporting the notion that the Sir4 N-terminus is a regulatory domain, we find it extensively phosphorylatedoncyclin-dependentkinaseconsensussites,somebeinghyperphosphorylatedduringmitosis.Mutationof two major phosphoacceptor sites (S63 and S84) derepresses natural subtelomeric genes when combined with a serendipitous mutation (P2A), which alone can enhance the stability of either the repressed or active state. The triple mutationconfersresistancetorapamycin-inducedstressandalossofsubtelomericrepression.WeconcludethattheSir4N- t