RNA-Seq Analysis of the Sclerotinia homoeocarpa – Creeping Bentgrass Pathosystem 英文参考文献.docVIP

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RNA-Seq Analysis of the Sclerotinia homoeocarpa – Creeping Bentgrass Pathosystem 英文参考文献.doc

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RNA-Seq Analysis of the Sclerotinia homoeocarpa – Creeping Bentgrass Pathosystem 英文参考文献

RNA-SeqAnalysisoftheSclerotiniahomoeocarpa– CreepingBentgrassPathosystem AngelaM.Orshinsky1*.,JinnanHu1.,StephenO.Opiyo2,VenuReddyvari-Channarayappa3, ThomasK.Mitchell1,MichaelJ.Boehm1 1DepartmentofPlantPathology, TheOhioStateUniversity,Columbus,Ohio,UnitedStatesofAmerica,2MolecularandCellularImagingCenter-Columbus.TheOhio StateUniversity,Columbus,Ohio,UnitedStatesofAmerica,3DaleBumpersNationalRiceResearchCenter,Stuttgart,Arizona,UnitedStatesofAmerica Abstract Sclerotinia homoeocarpa causes dollar spot disease, the predominate disease on highly-maintained turfgrass. Currently, therearemajorgapsinourunderstandingofthemolecularinteractionsbetweenS.homoeocarpaandcreepingbentgrass. In this study, 454 sequencing technology was used in the de novo assembly of S. homoeocarpa and creeping bentgrass transcriptomes. Transcript sequence data obtained using Illumina’s first generation sequencing-by-synthesis (SBS) were mapped to the transcriptome assemblies to estimate transcript representation in different SBS libraries. SBS libraries includedaS.homoeocarpaculturecontrol,acreepingbentgrassuninoculatedcontrol,andalibraryforcreepingbentgrass inoculatedwithS.homoeocarpaandincubatedfor96h.AFisher’sexacttestwasperformedtodeterminetranscriptsthat weresignificantlydifferentduringcreepingbentgrassinfectionwithS.homoeocarpa.Fungaltranscriptsofinterestincluded glycosyl hydrolases, proteases, and ABC transporters. Of particular interest were the large number of glycosyl hydrolase transcripts that target a wide range of plant cell wall compounds, corroborating the suggested wide host range and saprophytic abilities of S. homoeocarpa. Several of the multidrug resistance ABC transporters may be important for resistancetobothfungicidesandplantdefensecompounds.Creepingbentgrasstranscriptsofinterestincludedgermins, ubiquitintranscriptsinvolvedinproteasomedegradation,andcinnamoylreductase,whichisinvolvedinligninproduction. ThisanalysisprovidesanextensiveoverviewoftheS.homoeocarpa-turfgrassp