Ring-Like Distribution of Constitutive Heterochromatin in Bovine Senescent Cells 英文参考文献.docVIP

下载本文档

4
0
约 7页
2017-05-13 发布于上海
举报
版权申诉

Ring-Like Distribution of Constitutive Heterochromatin in Bovine Senescent Cells 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Ring-Like Distribution of Constitutive Heterochromatin in Bovine Senescent Cells 英文参考文献

Ring-LikeDistributionofConstitutiveHeterochromatin inBovineSenescentCells AndreyPichugin1,2,3*,NathalieBeaujean1,2,XavierVignon1,2,YegorVassetzky3* 1INRAUMR1198GroupeBiologieduDe′veloppementetReproduction,JouyenJosas,France,2ENVAUMR1198GroupeBiologieduDe′veloppementetReproduction, JouyenJosas,France,3CentreNationaldelaRechercheScientifiqueUMR8126,Universite′ Paris-Sud11,InstitutdeCance′rologieGustave-Roussy,Villejuif,France Abstract Background:Cellsthatreach‘‘Hayflicklimit’’ofproliferation,knownassenescentcells,possessaparticulartypeofnuclear architecture. Human senescent cells are characterized by the presence of highly condensed senescent associated heterochromatin foci (SAHF) that can be detected both by immunostaining for histone H3 three-methylated at lysine 9 (H3K9me3)andbyDAPIcounterstaining. Methods:Wehavestudiednucleararchitectureinbovinesenescentcellsusingacombinationofimmunofluorescenceand 3Dfluorescentin-situhybridization(FISH). Results: Analysis of heterochromatin distribution in bovine senescent cells using fluorescent in situ hybridization for pericentric chromosomal regions, immunostaining of H3K9me3, centromeric proteins CENP A/B and DNA methylation showedalowerlevelofheterochromatincondensationascomparedtoyoungcells.NoSAHFfociwereobserved.Instead, weobservedfibrousring-likeorribbon-likeheterochromatinpatternsthatwereundetectablewithDAPIcounterstaining. Theseheterochromatinfiberswereassociatedwithnucleoli. Conclusions:Constitutiveheterochromatininbovinesenescentcellsisorganizedinring-likestructures. Citation:PichuginA,BeaujeanN,VignonX,VassetzkyY(2011)Ring-LikeDistributionofConstitutiveHeterochromatininBovineSenescentCells.PLoSONE6(11): e26844.doi:10.1371/journal.pone.0026844 Editor:Pierre-AntoineDefossez,Universite′ Paris-Diderot,France ReceivedJuly8,2011;AcceptedOctober5,2011;PublishedNovember23,2011 Copyright: ? 2011 Pichugin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, whi