RINL, Guanine Nucleotide Exchange Factor Rab5-Subfamily, Is Involved in the EphA8-Degradation Pathway with Odin 英文参考文献.docVIP

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RINL, Guanine Nucleotide Exchange Factor Rab5-Subfamily, Is Involved in the EphA8-Degradation Pathway with Odin 英文参考文献.doc

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RINL, Guanine Nucleotide Exchange Factor Rab5-Subfamily, Is Involved in the EphA8-Degradation Pathway with Odin 英文参考文献

RINL,GuanineNucleotideExchangeFactor Rab5-Subfamily,IsInvolvedintheEphA8-Degradation PathwaywithOdin HiroakiKajiho.,ShinichiFukushima.,KenjiKontani,ToshiakiKatada* DepartmentofPhysiologicalChemistry,GraduateSchoolofPharmaceuticalSciences,UniversityofTokyo,Tokyo,Japan Abstract TheRabfamilyofsmallguanosinetriphosphatases(GTPases)playsavitalroleinmembranetrafficking.ItsactiveGTP-bound stateisdrivenbyguaninenucleotide-exchangefactors(GEFs).RasandRabinteractor(orRasinteraction/interference)-like (RINL),whichcontainsaconservedVPS9domaincriticalforGEFaction,wasrecentlyidentifiedasanewRab5subfamilyGEF invitro.However,itsdetailedfunctionandinteractingmoleculeshavenotyetbeenfullyelucidated.Herewefoundthat RINLhasGEFactivityfortheRab5subfamilyproteinsbymeasuringtheirGTP-boundformsinculturedcells.Wealsofound that RINL interacts with odin, a member of the ankyrin-repeat and sterile-alpha motif (SAM) domain-containing (Anks) protein family. In addition, the Eph tyrosine kinase receptor EphA8 formed a ternary complex with both RINL and odin. Interestingly,RINLexpressioninculturedcellsreducedEphA8levelsinamannerdependentonbothitsGEFactivityand interactionwithodin.Inaddition,knockdownofRINLincreasedEphA8levelinHeLacells.OurfindingssuggestthatRINL,as aGEFforRab5subfamily,isimplicatedintheEphA8-degradationpathwayviaitsinteractionwithodin. Citation:KajihoH,FukushimaS,KontaniK,KatadaT(2012)RINL,GuanineNucleotideExchangeFactorRab5-Subfamily,IsInvolvedintheEphA8-Degradation PathwaywithOdin.PLoSONE7(1):e30575.doi:10.1371/journal.pone.0030575 Editor:AnnaMariaDelprato,InstitutEurope′endeChimieetBiologie,France ReceivedApril25,2011;AcceptedDecember23,2011;PublishedJanuary23,2012 Copyright: ? 2012 Kajiho et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:Thisworkwassupportedinpartbyresearchgrants(toH.K.,K.K.an