蜂胶抑制变形链球菌生长和粘附的体外研究.pdfVIP

e京口腔医学 2002年 第10卷 第2期 · 79 蜂胶抑制变形链球菌生长和粘附的体外研究 王 晶① 李玉晶① 张春梅① 赵 静② 王风忠@ 摘 要 目的：研究蜂胶对变形链球菌生长和牯附的影响 方法：①抑菌实验；分别将水溶性蜂胶和醇溶性蜂胶提取物 等比稀释．采用钢管琼脂扩散法对变形链球菌和远缘链球菌做抑菌实验，测定最小抑菌浓度 ②粘附抑制实验：在含不同浓 度水溶性和醇溶性蜂胶，洗必泰．己醇的轻唾液体培养基中培养变形链球菌．8天后测定粘附于毛细管表面变形链球菌量，并 进行比较。结果：对变形链球菌和远绦链球菌最小抑菌浓度分别是：水溶性蜂胶为0．031625％和0．25％；醇溶性蜂胶均为 0．078％。蜂胶各组及洗必泰组均可显著抑制变形链球菌的粘附(P0．05)，0．5％水溶性蜂胶组，醇溶性蜂胶组及洗必泰组 抑制粘附作用更强，三组之间无显著性差异(P0．05)。结论：水溶性蜂胶和醇溶性蜂胶对变形链球菌生长和粘附都有显著 的抑制作用。 关键词 蜂胶 变形链球菌 远缘链球菌 生长 粘附 中围号 R378．1 R931．74 文献标识码 A lNVrrRo SrUDYON THE肼鲫 0F PR0PoL坞 ON T皿 GROWIItAND A1]m ㈣ oF S．MUTANS WANG Jing e1．a1．Faculty 0f Stornatulngy．CapitM Unlve~ty 0f Medi~l Sciences．Beijing 100050 A】晖IRACT 0bje嘶 To study the 比t of pmpofis∞growth and adh~enm of S mutans．Metlm~ (1)The experiment of inhibiting the growth。f earJogenic Mcteria惝 pe 鲫n。d．T minimum nhibitor v c~IomtratLon of progolis。【tracts to inhibit S murat~and S．sobrinus was assayed by rneans of using stairless-steel cylinder~with proportional diluted watermluble prop4is and ethamulic extract 0fprot~is(2)T ee~ rlr／ter／tofinhibitingthe adherenceofcariogenicbacteriaS．mutamwere culture continuous— ly in different MS liquid culture moditm~which contained various mncentration of watersoluble pmpulis．etharnolic extract nf progdls， cNorhexdine and ethanol After 8 days．theweight 0fthe cells ofS．mut~lswhicb ∞adhered or／the capillary 5u栅 w5measured and~mpamt R啪 lts The mirfimum inhibitary crmcentrations d pr。po extmc~to S rⅢltans and S sobrinus~vere 0．031625％、 0．25％ watemoluNe propulis．0．078％ ethamolic extract 0f pmpolis respectiveJy Each group ofPropolis ex~act and chlorhexdlne sJg— nificantlyinhibitedthe adherence of S．mutmas(尸0．05)The effect ofthe groups of 0．5％watersoluble propolis．ethamolic extmct nf propo[is and ch／orhexdi~e w stronger whereas there was no ignificant differences malong three groups(P0 05)．C