Role of Specific Quorum-Sensing Signals in the Regulation of Exopolysaccharide II Production within Sinorhizobium meliloti Spreading Colonies 英文参考文献.docVIP

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Role of Specific Quorum-Sensing Signals in the Regulation of Exopolysaccharide II Production within Sinorhizobium meliloti Spreading Colonies 英文参考文献.doc

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Role of Specific Quorum-Sensing Signals in the Regulation of Exopolysaccharide II Production within Sinorhizobium meliloti Spreading Colonies 英文参考文献

RoleofSpecificQuorum-SensingSignalsinthe RegulationofExopolysaccharideIIProductionwithin SinorhizobiummelilotiSpreadingColonies MengshengGao*,AndrewCoggin¤,KrutiYagnik,MaxTeplitski SoilandWaterScienceDepartment,UniversityofFlorida/InstituteofFoodandAgriculturalSciences,Gainesville,Florida,UnitedStatesofAmerica Abstract Background: Quorum sensing (QS) in Sinorhizobium meliloti involves at least half a dozen different N-acyl homoserine lactone (AHL) signals. These signals are produced by SinI, the sole AHL synthase in S. meliloti Rm8530. The sinI gene is regulated by two LuxR-type transcriptional regulators, SinR and ExpR. Mutations in sinI, sinR and expR abolish the productionofexopolysaccharideII(EPSII). Methodology/PrincipalFindings:ThisstudyinvestigatedanewtypeofcoordinatedsurfacespreadingofRm8530thatcan becategorizedasswarming.Motilityassaysonsemi-solidsurfacesrevealedthatbothflagellaandEPSIIarerequiredforthis typeofmotility.TheproductionofEPSIIdependsonAHLsproducedbySinI.OftheseAHLs,onlyC16:1-and3-oxo-C16:1- homoserine lactones(HSLs)stimulatedswarmingin anExpR-dependent manner.ThesetwoAHLsinducedthestrongest responseinthewggRreporterfusions.WggRisapositiveregulatoroftheEPSIIbiosynthesisgeneexpression.Thelevelsof thewggRactivationcorrelatedwiththeextentofswarming.Furthermore,swarmingofS.melilotirequiredthepresenceof thehighmolecularweight(HMW)fractionofEPSII.Withinswarmingcolonies,arecombinase-basedRIVETreporterinthe wggRgenewasresolvedin30%ofthecells,indicatinganenhancedregulationofEPSIIproductioninthesubpopulationof cells,whichwassufficienttosupportswarmingoftheentirecolony. Conclusions/Significance:SwarmingbehaviorofS.melilotiRm8530onsemi-solidsurfacesisfoundtobedependentonthe functional QS regulatory cascades. Even though multiple AHL signals are produced by the bacterium, only two AHLs species,C16:1-and3-oxo-C16:1-HSLs,affectedswarmingbyup-regulatingtheexpressionofwggR.WhileEPSIIisproducedby Rm8530 as high and low molecular weight fractions, only the HMW