Role of Spectraplakin in Drosophila Photoreceptor Morphogenesis 英文参考文献.docVIP

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Role of Spectraplakin in Drosophila Photoreceptor Morphogenesis 英文参考文献.doc

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RoleofSpectraplakininDrosophilaPhotoreceptor Morphogenesis UyenNgocMui,ChristinaM.Lubczyk,Sang-ChulNam* DepartmentofBiology,BaylorUniversity,Waco,Texas,UnitedStatesofAmerica Abstract Background: Crumbs (Crb), a cell polarity gene, has been shown to provide a positional cue for the apical membrane domain and adherens junction during Drosophila photoreceptor morphogenesis. It has recently been found that stable microtubulesindevelopingDrosophilaphotoreceptorswerelinkedtoCrblocalization.Coordinatedinteractionsbetween microtubuleandactincytoskeletonsareinvolvedinmanypolarizedcellularprocesses.SinceSpectraplakinisabletobind bothmicrotubuleandactincytoskeletons,theroleofSpectraplakinwasanalyzedintheregulationsofapicalCrbdomainin developingDrosophilaphotoreceptors. Methodology/PrincipalFindings:ThelocalizationpatternofSpectraplakinindevelopingpupalphotoreceptorsshoweda uniqueintracellulardistribution.Spectraplakinlocalizedatrhabdomereterminalwebwhichisatthebasalsideoftheapical Crb or rhabdomere, and in between the adherens junctions. The spectraplakin mutant photoreceptors showed dramatic mislocalizations of Crb, adherens junctions, and the stable microtubules. This role of Spectraplakin in Crb and adherens junctionregulationwasfurthersupportedbyspectraplakin’sgain-of-functionphenotype.Spectraplakinoverexpressionin photoreceptorscausedacellpolaritydefectincludingdramaticmislocalizationofCrb,adherensjunctionsandthestable microtubulesinthedevelopingphotoreceptors.Furthermore,astronggeneticinteractionbetweenspectraplakinandcrb wasfoundusingageneticmodifiertest. Conclusions/Significance:Insummary,wefoundauniquelocalizationofSpectraplakininphotoreceptors,andidentified the role of spectraplakin in the regulation of the apical Crb domain and adherens junctions through genetic mutational analysis. Our data suggest that Spectraplakin, an actin-microtubule cross-linker, is essential in the apical and adherens junctioncontrolsduringthephotoreceptorsmorphogenesis. Citation: Mui UN,