Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana 英文参考文献.docVIP

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Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana 英文参考文献.doc

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Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana 英文参考文献

SingleNucleotidePolymorphismTypingof MycobacteriumulceransRevealsFocalTransmissionof BuruliUlcerinaHighlyEndemicRegionofGhana KatharinaRo¨ltgen1,2,WeihongQi1,2¤a,Marie-The′re`seRuf1,2,ErnestinaMensah-Quainoo3,SachaJ. Pidot4,TorstenSeemann5,TimothyP.Stinear4,MichaelKa¨ser1,2¤b,DorothyYeboah-Manu6 ,Gerd Pluschke1,2 * 1SwissTropicalandPublicHealthInstitute,MolecularImmunology,Basel,Switzerland,2UniversityofBasel,Basel,Switzerland,3GhanaHealthService,MinistryofHealth, Tema, Ghana, 4Department of Microbiology, Monash University, Clayton, Australia, 5Victorian Bioinformatics Consortium, Monash University, Clayton, Australia, 6NoguchiMemorialInstituteforMedicalResearch,UniversityofGhana,Legon,Ghana Abstract Buruliulcer(BU)isanemergingnecrotizingdiseaseoftheskinandsubcutaneoustissuecausedbyMycobacteriumulcerans. WhileproximitytostagnantorslowflowingwaterbodiesisariskfactorforacquiringBU,theepidemiologyandmodeofM. ulceranstransmissionispoorlyunderstood.Herewehaveusedhigh-throughputDNAsequencingandcomparisonsofthe genomesof seven M.ulcerans isolatesthat appeared monomorphic by existing typing methods. We identified alimited number of single nucleotide polymorphisms (SNPs) and developed a real-time PCR SNP typing method based on these differences.WetheninvestigatedclinicalisolatesofM.ulceransonwhichwehaddetailedinformationconcerningpatient locationandtimeofdiagnosis.WithintheDensuriverbasinofGhanaweobserveddominanceofoneclonalcomplexand localclusteringofsomeofthevariantsbelongingtothiscomplex.Theseresultsrevealfocaltransmissionanddemonstrate, thatmicro-epidemiologicalanalysesbySNPtypinghasgreatpotentialtohelpusunderstandhowM.ulceransistransmitted. Citation:Ro¨ltgenK,QiW,RufM-T,Mensah-QuainooE,PidotSJ,etal.(2010)SingleNucleotidePolymorphismTypingofMycobacteriumulceransRevealsFocal TransmissionofBuruliUlcerinaHighlyEndemicRegionofGhana.PLoSNeglTropDis4(7):e751.doi:10.1371/journal.pntd.0000751 Editor:MathieuPicardeau,InstitutPasteur,France ReceivedJanuary11,2010;AcceptedJune3,201