SINE RNA Induces Severe Developmental Defects in Arabidopsis thaliana and Interacts with HYL1 (DRB1), a Key Member of the DCL1 Complex 英文参考文献.docVIP

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SINE RNA Induces Severe Developmental Defects in Arabidopsis thaliana and Interacts with HYL1 (DRB1), a Key Member of the DCL1 Complex 英文参考文献.doc

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SINE RNA Induces Severe Developmental Defects in Arabidopsis thaliana and Interacts with HYL1 (DRB1), a Key Member of the DCL1 Complex 英文参考文献

SINERNAInducesSevereDevelopmentalDefectsin ArabidopsisthalianaandInteractswithHYL1(DRB1),a KeyMemberoftheDCL1Complex Marie-Noe¨llePouch-Pe′lissier1.,ThierryPe′lissier1.,TalineElmayan2,Herve′ Vaucheret2,DraskoBoko3, MichaelF.Jantsch3,Jean-MarcDeragon1* 1Universite′ de Perpignan Via Domitia, CNRS UMR5096 LGDP, Perpignan, France, 2INRA Laboratoire de Biologie Cellulaire, Versailles, France, 3Department of ChromosomeBiology,MaxF.PerutzLaboratories,UniversityofVienna,Vienna,Austria Abstract The proper temporal and spatial expression of genes during plant development is governed, in part, by the regulatory activities of various types of small RNAs produced by the different RNAi pathways. Here we report that transgenic ArabidopsisplantsconstitutivelyexpressingtherapeseedSB1SINEretroposonexhibitdevelopmentaldefectsresembling thoseobservedinsomeRNAimutants.WeshowthatSB1RNAinteractswithHYL1(DRB1),adouble-strandedRNA-binding protein(dsRBP)thatassociateswiththeDicerhomologueDCL1toproducemicroRNAs.RNaseV1protectionassaysmapped thebindingsiteofHYL1toaSB1regionthatmimicsthehairpinstructureofmicroRNAprecursors.WealsoshowthatHYL1, upon binding to RNA substrates, induces conformational changes that force single-stranded RNA regions to adopt a structuredhelix-likeconformation.XenopuslaevisADAR1,butnotArabidopsisDRB4,bindsSB1RNAinthesameregionas HYL1,suggestingthatSINERNAsbindonlyasubsetofdsRBPs.Consistently,DCL4-DRB4-dependentmiRNAaccumulation was unchanged in SB1 transgenic Arabidopsis, whereas DCL1-HYL1-dependent miRNA and DCL1-HYL1-DCL4-DRB4- dependent tasiRNA accumulation was decreased. We propose that SINE RNA can modulate the activity of the RNAi pathwaysinplantsandpossiblyinothereukaryotes. Citation:Pouch-Pe′lissierM-N,Pe′lissierT,ElmayanT,VaucheretH,BokoD,etal.(2008)SINERNAInducesSevereDevelopmentalDefectsinArabidopsisthaliana andInteractswithHYL1(DRB1),aKeyMemberoftheDCL1Complex.PLoSGenet4(6):e1000096.doi:10.1371/journal.pgen.1000096 Editor:GregoryP.Copenhaver,TheUniver