Simultaneous Measurement of Neural Spike Recordings and Multi-Photon Calcium Imaging in Neuroblastoma Cells 英文参考文献.docVIP

Sensors 2012, 12, 15281-15291; doi:10.3390/s121115281 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Simultaneous Measurement of Neural Spike Recordings and Multi-Photon Calcium Imaging in Neuroblastoma Cells Suhwan Kim 1, Unsang Jung 1, Juyeong Baek 1, Shinwon Kang 2,* and Jeehyun Kim 2,* 1 School of Electrical Engineering and Computer Science, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Korea; E-Mails: shkim@ee.knu.ac.kr (S.K.); cester@ (U.J.); young02you@ (J.B.) 2 School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Korea * Authors to whom correspondence should be addressed; E-Mails: swkang@knu.ac.kr (S.K.); jeehk@knu.ac.kr (J.K.); Tel./Fax: +82-53-950-7221. Received: 9 August 2012; in revised form: 5 November 2012 / Accepted: 6 November 2012 / Published: 8 November 2012 Abstract: This paper proposes the design and implementation of a micro-electrode array (MEA) for neuroblastoma cell culturing. It also explains the implementation of a multi-photon microscope (MPM) customized for neuroblastoma cell excitation and imaging under ambient light. Electrical signal and fluorescence images were simultaneously acquired from the neuroblastoma cells on the MEA. MPM calcium images of the cultured neuroblastoma cell on the MEA are presented and also the neural activity was acquired through the MEA recording. A calcium green-1 (CG-1) dextran conjugate of 10,000 D molecular weight was used in this experiment for calcium imaging. This study also evaluated the calcium oscillations and neural spike recording of neuroblastoma cells in an epileptic condition. Based on our observation of neural spikes in neuroblastoma cells with our proposed imaging modality, we report that neuroblastoma cells can be an important model for epileptic activity studies. Keywords: micro-electrode