Simultaneous Determination of Flavonoids in Different Parts of Citrus reticulata ‘Chachi’ Fruit by High Performance Liquid Chromatography—Photodiode Array Detection 英文参考文献.docVIP

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Simultaneous Determination of Flavonoids in Different Parts of Citrus reticulata ‘Chachi’ Fruit by High Performance Liquid Chromatography—Photodiode Array Detection 英文参考文献.doc

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Simultaneous Determination of Flavonoids in Different Parts of Citrus reticulata ‘Chachi’ Fruit by High Performance Liquid Chromatography—Photodiode Array Detection 英文参考文献

Molecules 2010, 15, 5378-5388; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Simultaneous Determination of Flavonoids in Different Parts of Citrus reticulata ‘Chachi’ Fruit by High Performance Liquid Chromatography—Photodiode Array Detection Yinshi Sun 1, Jianhua Wang 1,*, Shubo Gu 1, Zhengbo Liu 2, Yujie Zhang 2 and Xiaoxia Zhang 2 1 State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, 271018, China 2 Agricultural Science Research Institute of Shandong, Taian, 271000, China * Author to whom correspondence should be addressed; E-Mail: sdauwangjh@163.com; Tel.: +86 538 8242226; Fax: +86 538 8242226. Received: 12 July 2010; in revised form: 27 July 2010 / Accepted: 2 August 2010 / Published: 5 August 2010 Abstract: Flavonoids are important polyphenolic secondary metabolites in plant. Citrus reticulata ‘Chachi’ fruit are rich in flavonoids and are being used as functional antioxidant ingredients for the treatment of atherosclerosis and cancer, etc. A high performance liquid chromatography-photodiode array detection system was used to analyze five flavonoids, namely, naringin, hesperidin, didymin, tangeretin and nobiletin, in different parts of C. reticulata ‘Chachi’ fruit. The chromatographic analysis was performed on a C18 column with a gradient elution of acetonitrile and water at a flow rate of 1.0 mL/min. Detection was carried out using a photodiode array detector at 280 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (R2 0.9995). Precision and reproducibility were evaluated by six replicated analyses, and the R.S.D. values were less than 0.9% and 2.7%. The recoveries were between 98.37 and 103.89%. This method is promising to improve the quality control of different parts of C. reticulata ‘Chachi’ fruit. Keywords: high per