Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study 英文参考文献.docVIP

下载本文档

15
0
约2.05万字
约 6页
2017-05-13 发布于上海
举报
版权申诉

Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study 英文参考文献

Chenetal.ChineseMedicine2010,5:12 /content/5/1/12 RESEARCH OpenAccess Simultaneousdeterminationofthreemajor bioactivesaponinsofPanaxnotoginsengusing liquidchromatography-tandemmass spectrometryandapharmacokineticstudy WeiChen,YunjieDang,ChunyanZhu* Abstract Background:Panaxnotoginsengsaponins(PNS),themainactivecomponentsofRadixNotoginseng,hasbeen usedfortreatingatherosclerosis,cerebralinfarction,andcerebralischemia.GinsenosidesRg1,ginsenosideRb1 ,and notoginsenosideR1arethemaincontributorsofbiologicalactivities,determinationofthesethreesaponinsisvery importantfortheinvivoevaluationofPNS.Thepresentstudyaimstodevelopaliquidchromatography-tandem massspectrometry(LC-MS/MS)methodforthesimultaneousquantificationofginsenosidesRg1,ginsenosideRb1, andnotoginsenosideR1.Theuseofthismethodwasexemplifiedinpharmacokineticstudyofbeagledogplasma afteroraladministrationofPNS. Methods:Liquidchromatography-tandemmassspectrometry(LC/MS/MS)methodwascombinedwithsolid-phase extraction(SPE).ThissetupwasusedtodeterminesimultaneouslythethreemajorPNS(ginsenosideRg1, ginsenosideRb1,andnotoginsenosideR1)inbeagledogplasma.Tandemmassspectrometrywasperformedusing electrosprayionizationinthepositiveionmode. Results:Thelowerlimitsofquantificationwere0.5ng/mLfornotoginsenosideR1,0.82ng/mLforginsenosideRg1, and1.10ng/mLforginsenosideRb1.Thecalibrationcurvesforthethreesaponinswerelinearoverthe 2 2 concentrationranges2.64-264ng/mL(r =0.9967,P=0.003),3.6-360ng/mL(r =0.9941,P=0.004),and18.7-1870 ng/mL(r2=0.9912,P=0.004)fornotoginsenosideR1,ginsenosideRg1,andginsenosideRb1,respectively.Within theseconcentrationranges,therelativestandarddeviation(RSD)ofintra-andinterdayassaysforthethreePNS frombeagledogplasmasampleswerelessthan12%. Conclusions:ThisLC/MS/MSmethodincombinationwithSPEisusefulinthepharmacokineticstudyofPNS,such asthesimultaneousdeterminationofsaponinsinbeagledogplasmaafteroraladministration. Background threesaponinsisveryimportantfortheinvivomonitor- Panax