Simultaneous Determination of Salidroside and Its Aglycone Metabolite p-Tyrosol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry 英文参考文献.docVIP

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Simultaneous Determination of Salidroside and Its Aglycone Metabolite p-Tyrosol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry 英文参考文献.doc

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Simultaneous Determination of Salidroside and Its Aglycone Metabolite p-Tyrosol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry 英文参考文献

Molecules 2012, 17, 4733-4754; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Simultaneous Determination of Salidroside and Its Aglycone Metabolite p-Tyrosol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry Na Guo 1,?, Zhiwei Hu 1,?, Xiaoxu Fan 2, Jian Zheng 1, Dehui Zhang 1, Tao Xu 1, Tao Yu 1, Yang Wang 1,* and Haiying Li 2,* 1 Alkali Soil Natural Environmental Science Center, Northeast Forestry University/Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China; E-Mails: guona0329@126.com (N.G.); huzhiwei.haerbin@ (Z.H.) 2 College of Life Sciences, Heilongjiang University, Harbin 150080, China ? These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: ywang@ (Y.W.); lvzh3000@ (H.L.); Tel./Fax: +86-451-8219-0052 (Y.W.). Received: 17 January 2012; in revised form: 3 April 2012 / Accepted: 13 April 2012 / Published: 23 April 2012 Abstract: Salidroside and its aglycone p-tyrosol are two major phenols in the genus Rhodiola and have been confirmed to possess various pharmacological properties. In our present study, p-tyrosol was identified as the deglycosylation metabolite of salidroside after intravenous (i.v.) administration to rats at a dose of 50 mg/kg, but was not detectable after intragastric gavage (i.g.) administration through HPLC-photodiode array detection (PDA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Next, an accurate and precise LC-MS/MS method was developed to quantitatively determine salidroside and p-tyrosol in rat plasma samples. Samples were analyzed by LC-MS/MS on a reverse-phase xTerra MS C18 column which was equilibrated and eluted with an isocratic mixture of acetonitrile-water (1:9, v/v) at a flow rate of 0.3 mL/min. The analytes were monitored by multiple reaction mon