Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography 英文参考文献.docVIP

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Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography 英文参考文献.doc

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Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography 英文参考文献

Chinese Medicine BioMedCentral Research Open Access Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography Jingzheng Song, Quanbin Han, Chunfeng Qiao, Yuekeung Yip and Hongxi Xu* Address: Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, NT, Hong Kong SAR, China Email: Jingzheng Song - jzsong@; Quanbin Han - qbhan@; Chunfeng Qiao - cfqiao@; Yuekeung Yip - masonyip@; Hongxi Xu* - xuhongxi@ * Corresponding author Published: 20 June 2007 Received: 1 September 2006 Accepted: 20 June 2007 Chinese Medicine 2007, 2:7 doi:10.1186/1749-8546-2-7 This article is available from: /content/2/1/7 ? 2007 Song et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Concentrated Gegen Tang (GT) granule is a widely available traditional Chinese medicinal product for the treatment of cold and flu. There was no reliable analytical method available for the quality assessment of GT granules. Methods: An HPLC method with an Agilent Zorbax SB-Phenyl Stablebond column (250 × 4.6 mm, 5 μm) was developed and validated. The mobile phase gradient was a mixture of 0.1% trifluoroacetic acid (TFA) in acetonitrile (ACN) and 0.1% TFA in water. The detection with a diode- array detector was set at 207, 230, 250 and 275 nm. Seven components, namely puerarin, daidzein, paeoniflorin, cinnamic acid, glycyrrhizin, ephedrine and pseudoephedrine were selected as marker compounds for the evaluation. Results: The regression equations revealed good linear relationships (correlation coefficients: 0.9994–0.9998) between the peak areas and concentrations. The recovery was between 98.