Spontaneous sister chromatid exchanges in mitotic chromosomes of cattle (Bos taurus L) 英文参考文献.docVIP

Spontaneous sister chromatid exchanges in mitotic chromosomes of cattle (Bos taurus L) 1 D Di 1 V Jovino P Marigliano 3 ‘Federico II’, Department of Animal Science, 80055 Portici, Naples; 2 (Received 9 September 1994; accepted 12 May 1995) Summary - Peripheral blood lymphocytes from 4 cattle donors were grown for 2 cell cycles in the presence of 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0 wg/ml of 5-bromodeoxyuridine in order to detect the spontaneous level of sister chromatid exchanges and the dose-response relationships at low bromodeoxyuridine levels. Sister chromatid exchanges were counted on 50 s cycle metaphase spreads, randomly scored, for each donor at each dose. The mean rate of sister chromatid exchanges/cell at 0.1 !Lg/ml of BUdR was 2.48 f 1.75, which can be considered as spontaneous. Thedose-response curve increased more rapidly from 0.1 to 0.5 !g/ml of bromodeoxyuridine and less rapidly at higher concentrations. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various to 1.0 wg/ml of bromodeoxyuridine. The usefulness of determining the spontaneous level of sister chromatid exchanges in domestic animals is discussed in relation to its possible application for selection programs. Résumé - Estimation des échanges interchromatidiques spontanés dans les chro- mosomes mitotiques de bovin (Bos taurus L). Des cultures de lymphocytes obtenus J de bromodéoxyuridine Nous avons étudié les relations réponse (échanges chromatidiques)- dose de BrdU afin, notamment, de conna?tre le niveau spontané des échanges chromatidiques chez cette espèce. Ces échanges ont été comptés sur 50 plaques en métaphase II (par donneur et par dose) choisis par hasard. Le taux moyen d’échanges interchromatidiques (SCE : sister chromatid exchanges) par cellule (rapport SCE/cellule) pour une concentration de 0,1 1 Mg/ml de BrdU est de comme le niveau spontané des échanges. La courbe réponse-dose de BrdU présente une  rapide augme