Studies on the Genetic Variation of the Green Unicellular Alga Haematococcus pluvialis (Chlorophyceae) Obtained from Different Geographical Locations Using ISSR and RAPD Molecular Marker 英文参考文献.docVIP

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Studies on the Genetic Variation of the Green Unicellular Alga Haematococcus pluvialis (Chlorophyceae) Obtained from Different Geographical Locations Using ISSR and RAPD Molecular Marker 英文参考文献.doc

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Studies on the Genetic Variation of the Green Unicellular Alga Haematococcus pluvialis (Chlorophyceae) Obtained from Different Geographical Locations Using ISSR and RAPD Molecular Marker 英文参考文献

Molecules 2011, 16, 2598-2608; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Studies on the Genetic Variation of the Green Unicellular Alga Haematococcus pluvialis (Chlorophyceae) Obtained from Different Geographical Locations Using ISSR and RAPD Molecular Marker Noroozi Mostafa 1,2, Hishamuddin Omar 1, Soon Guan Tan 3 and Suhaimi Napis 3,* 1 Department of Biology, Faculty of Science, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2 Department of Biology Faculty of Science, Alzahra University, Tehran 1993891176, Iran 3 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia * Author to whom correspondence should be addressed; E-Mail: suhaimi@.my; Tel.: +603or +603 Fax: +603 Received: 24 January 2011; in revised form: 1 March 2011 / Accepted: 4 March 2011 / Published: 22 March 2011 Abstract: Haematococcus pluvialis (Flotow) is a unicellular green alga, which is considered to be the best astaxanthin-producing organism. Molecular markers are suitable tools for the purpose of finding out genetic variations in organisms; however there have been no studies conducted on ISSR or RAPD molecular markers for this organism. The DNA of 10 different strains of H. pluvialis (four strains from Iran, two strains from Finland, one strain from Switzerland and three strains from the USA) was extracted. A genetic similarity study was carried out using 14 ISSR and 12 RAPD primers. Moreover, the molecular weights of the bands produced ranged from 0.14 to 3.4 Kb. The PCA and dendrogram clustered the H. pluvialis strains into various groups according to their geographical origin. The lowest genetic similarity was between the Iran2 and USA2 strains (0.08) and the highest genetic similarity was between Finland1 and