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Study of probe-sample distance for biomedical spectra measurement 英文参考文献
Wangetal.BioMedicalEngineeringOnLine2011,10:95
/content/10/1/95
RESEARCH
OpenAccess
Studyofprobe-sampledistanceforbiomedical
spectrameasurement
BowenWang1*,ShuzhenFan2,LeiLi2andCongWang2
*Correspondence:jnwbw@163.
Abstract
com
1HospitalofShandongUniversity,
No.91,ShandaBeiRoad,Jinan,
250100,P.R.China
Background:Fiber-basedopticalspectroscopyhasbeenwidelyusedforbiomedical
applications.However,theeffectofprobe-sampledistanceonthecollection
Fulllistofauthorinformationis
availableattheendofthearticle
efficiencyhasnotbeenwellinvestigated.
Method:Inthispaper,wepresentedatheoreticalmodeltomaximizethe
illuminationandcollectionefficiencyindesigningfiberopticprobesforbiomedical
spectrameasurement.Thismodelwasingeneralapplicabletoprobeswithsingleor
multiplefibersatanarbitraryincidentangle.Inordertodemonstratethetheory,a
fluorescencespectrometerwasusedtomeasurethefluorescenceofhumanfinger
skinatvariousprobe-sampledistances.Thefluorescencespectrumandthetotal
fluorescenceintensitywererecorded.
Results:Thetheoreticalresultsshowthatforsinglefiberprobes,contact
measurementalwaysprovidesthebestresults.Whileformulti-fiberprobes,thereis
anoptimalprobedistance.Whena400-μmexcitationfiberisusedtodeliverthe
lighttotheskinandanothersix400-μmfiberssurroundingtheexcitationfiberare
usedtocollectthefluorescencesignal,theexperimentalresultsshowthathuman
fingerskinhasverystrongfluorescencebetween475nmand700nmunder450nm
excitation.Thefluorescenceintensityisheavilydependentontheprobe-sample
distanceandthereisanoptimalprobedistance.
Conclusions:Weinvestigatedanumberofprobe-sampleconfigurationsandfound
thatcontactmeasurementcouldbetheprimarychoiceforsingle-fiberprobes,but
wasveryinefficientformulti-fiberprobes.Therewasanoptimalprobe-sample
distanceformulti-fiberprobes.Bycarefullychoosingtheprobe-sampledistance,the
collectionefficiencycouldbeenhancedby5-10times.Ourexperiments
demonstratedthattheexperimentalresultsoftheprobe-sampledistance
dependenceofcollectionefficiencyinmulti-fiberprobeswereingene
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