SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC) identification of early mediators of insect responses 英文参考文献.docVIP

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SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC) identification of early mediators of insect responses 英文参考文献.doc

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SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC) identification of early mediators of insect responses 英文参考文献

Gilardonietal.BMCPlantBiology2010,10:66 /1471-2229/10/66 RESEARCH ARTICLE OpenAccess SuperSAGEanalysisoftheNicotianaattenuata transcriptomeafterfattyacid-aminoacid elicitation(FAC):identificationofearlymediators ofinsectresponses PaolaAGilardoni1,StefanSchuck1,RuthJüngling2,Bj?rnRotter2,IanTBaldwin1,GustavoBonaventure1* Abstract Background:Plantstriggerandtailordefenseresponsesafterperceptionoftheoralsecretions(OS)ofattacking specialistlepidopteranlarvae.Fattyacid-aminoacidconjugates(FACs)intheOSoftheManducasextalarvaeare necessaryandsufficienttoelicittheherbivory-specificresponsesinNicotianaattenuata,anannualwildtobacco species.HowFACsareperceivedandactivatesignaltransductionmechanismsisunknown. Results:WeusedSuperSAGEcombinedwith454sequencingtoquantifytheearlytranscriptionalchangeselicited bytheFACN-linolenoyl-glutamicacid(18:3-Glu)andvirusinducedgenesilencing(VIGS)toexaminethefunction ofcandidategenesintheM.sexta-N.attenuatainteraction.TheanalysistargetedmRNAsencodingregulatory components:raretranscriptswithveryrapidFAC-elicitedkinetics(increaseswithin60anddeclineswithin120min). From12,744uniqueTagsequencesidentified(UniTags),430and117weresignificantlyup-anddown-regulated≥ 2.5-fold,respectively,after18:3-Gluelicitationcomparedtowounding.Basedongeneontologyclassification,more than25%oftheannotatedUniTagscorrespondedtoputativeregulatorycomponents,including30transcriptional regulatorsand22proteinkinases.QuantitativePCRanalysiswasusedtoanalyzetheFAC-dependentregulationof asubsetof27oftheseUniTagsandformostofthemarapidandtransientinductionwasconfirmed.SixFAC- regulatedgeneswerefunctionallycharacterizedbyVIGSandtwo,aputativelipidphosphatephosphatase(LPP) andaproteinofunknownfunction,wereidentifiedasimportantmediatorsoftheM.sexta-N.attenuatainteraction. Conclusions:TheanalysisoftheearlychangesinthetranscriptomeofN.attenuataafterFACelicitationusing SuperSAGE/454hasidentifiedregulatorygenesinvolvedininsect-specificmediatedresponsesinplants.Moreover, ithasprovidedafound