Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar 英文参考文献.docVIP

Dafoeetal.BMCPlantBiology2010,10:191 /1471-2229/10/191 RESEARCH ARTICLE OpenAccess Thaumatin-likeproteinsaredifferentially expressedandlocalizedinphloemtissuesof hybridpoplar NicoleJDafoe1,2,3,BrentEGowen1,CPeterConstabel1,2* Abstract Background:Twothaumatin-likeproteins(TLPs)werepreviouslyidentifiedinphloemexudateofhybridpoplar (Populustrichocarpa×P.deltoides)usingproteomicsmethods,andtheirsieveelementlocalizationconfirmedby immunofluorescence.Inthecurrentstudy,weanalyzeddifferenttissuestofurtherunderstandTLPexpressionand localizationinpoplar,andusedimmunogoldlabellingtodetermineintracellu