The uses of genome-wide yeast mutant collections 英文参考文献.docVIP

Review The uses of genome-wide yeast mutant collections Bart Scherens* and Andre Goffeau? Addresses: *Institut de Recherches Microbiologiques J.M. Wiame, Campus CERIA, Av. E. Gryson 1, 1070 Bruxelles, Belgium. ?Institut des Sciences de la Vie, Université Catholique de Louvain, Croix du Sud 2-20, 1348 Louvain-la-Neuve, Belgium. Correspondence: Andre Goffeau. E-mail: goffeau@fysa.ucl.ac.be Published: 25 June 2004 Genome Biology 2004, 5:229 The electronic version of this article is the complete one and can be found online at /2004/5/7/229 ? 2004 BioMed Central Ltd Abstract We assess five years of usage of the major genome-wide collections of mutants from Saccharomyces cerevisiae: single deletion mutants, double mutants conferring ‘synthetic’ lethality and the ‘TRIPLES’ collection of mutants obtained by random transposon insertion. Over 100 experimental conditions have been tested and more than 5,000 novel phenotypic traits have been assigned to yeast genes using these collections. In April 1996, the completely annotated genome sequence of the yeast Saccharomyces cerevisiae was made publicly available [1,2], the first eukaryotic genome sequence to be completed. Eight years later, thanks to the united efforts of the large yeast research community and to the unique genetic and physiological properties of yeast, this humble servant of mankind provides by far the best annotated eukaryotic genome [3]. The completeness of the yeast genome sequence has allowed the development of many novel tools for analyzing all molecular components of the cell and their interactions. These tools include three high- throughput collections of mutants that were first produced in 1999 and that have been analyzed in the five years since then. Here, we review the uses of these collections and their contribution to the identification of the components of b