Three New Oblongolides from Phomopsis sp. XZ-01, an Endophytic Fungus from Camptotheca acuminate 英文参考文献.docVIP

Molecules 2011, 16, 3351-3359; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Three New Oblongolides from Phomopsis sp. XZ-01, an Endophytic Fungus from Camptotheca acuminate Ting Lin 1,?,*, Guang Hui Wang 1,?, Xiang Lin 2, Zhi Yu Hu 3, Quan Cheng Chen 1, Yang Xu 1, Xiao Kun Zhang 1 and Hai Feng Chen 1,* 1 School of Pharmaceutical Sciences, Xiamen University, 422 South Siming Road, Xiamen, Fujian 361005, China; E-Mail: guanghui@ (G.H.W.); chenqc@ (Q.C.C.); xu_yang@ (Y.X.); xzhang@ (X.K.Z.), 2 Institute of Life Sciences, Fuzhou University, No.2 Xueyuan Rd., University Town, Fuzhou, Fujian 350108, China; E-Mail: linx1981@ (X.L.) 3 School of Life Sciences, Xiamen University, Fujian 361005, China; E-Mail: huzhiyu@ (Z.Y.H.) ? These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: linting@ (T.L.); haifeng@ (H.F.C.); Tel.: +86-592-2187225; Fax: +86-592-2181879. Received: 17 March 2011; in revised form: 1 April 2011 / Accepted: 2 April 2011 / Published: 19 April 2011 Abstract: Four new metabolites, including three new oblongolides named C1, P1, and X1 (1-3) and 6-hydroxyphomodiol (10), along with eight known compounds – oblongolides B (4), C (5), D (6), O (7), P (8) and U (9), (3R,4aR,5S,6R)-6-hydroxy-5-methylramulosin (11), and (3R)-5-methylmellein (12) – were isolated from the endophytic fungal strain Phomopsis sp. XZ-01 of Camptotheca acuminate. Their structures were elucidated by spectroscopic analyses, including 1H- and 13C-NMR, 2D NMR (HSQC, HMBC, 1H-1H COSY and NOESY) and HR-FT-MS. Cytotoxic activities of these compounds were evaluated. Some of them showed weak selective activities. Keywords: Camptotheca acuminate; endophytic fungus; Phomopsis sp. XZ-01; oblongolides; new metabolites  Molecules 2011, 16 3352 1. Introduction Endophytes, especially those found in medicinal plants,