Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles 英文参考文献.docVIP

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Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles 英文参考文献.doc

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Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles 英文参考文献

Sensors 2011, 11, 11335-11342; doi:10.3390/s111211335 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles Harri H?rm? 1,*, Juha Toivonen 1,2, Juhani T. Soini 1,3, Pekka H?nninen 1 and Wolfgang J. Parak 4 1 Laboratory of Biophysics, University of Turku, 20520 Turku, Finland; E-Mails: juhani.soini@utu.fi (J.S.); pekka.hanninen@utu.fi (P.H.) 2 Department of Physics, Tampere University of Technology, 33101 Tampere, Finland; E-Mail: juha.toivonen@tut.fi (J.T.) 3 Turku University of Applied Sciences, 20520 Turku, Finland 4 Fachbereich Physik and Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universit?t Marburg., 35037 Marburg, Germany; E-Mail: wolfgang.parak@physik.uni-marburg.de (W.P.) * Author to whom correspondence should be addressed; E-Mail: harri.harma@utu.fi; Tel.: +358-2-333-7065; Fax: +358-2-333-8413. Received: 24 August 2011; in revised form: 18 October 2011 / Accepted: 14 November 2011 / Published: 28 November 2011 Abstract: Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation. Keywords: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bi