Trametes sp. as a Source of Biopolymer Cross-Linking Agents Laccase Induced Gelation of Ferulated Arabinoxylans 英文参考文献.docVIP

Molecules 2009, 14, 4159-4165; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans Alva Castillo 1, Agustín Rascón-Chu 2,*, Georgina Vargas 2, Elizabeth Carvajal-Millán 3, Elisa Valenzuela-Soto 4, Rogerio R. Sotelo-Mundo 3 and Ana Luisa Martínez 3 1 Facultad de Zootecnia, Universidad Autónoma de Chihuahua, Chihuahua, Chihuahua 31120, Mexico 2 Research Center for Food and Development, CIAD, A. C, DTAOV, Hermosillo, Sonora 83000, Mexico 3 Research Center for Food and Development, CIAD, A. C, DTAOA, Hermosillo, Sonora 83000, Mexico 4 Research Center for Food and Development, CIAD, A. C, DCA, Hermosillo, Sonora 83000, Mexico * Author to whom correspondence should be addressed; E-Mail: arascon@ciad.mx. Received: 10 September 2009; in revised form: 16 October 2009 / Accepted: 16 October 2009 / Published: 16 October 2009 Abstract: This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 ?g/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase. Keywords: polysaccharide; cross-linking; ferulic acid; viscosity; gels  Molecules 2009, 14 4160 1. Introd