Transcription, one allele at a time 英文参考文献.docVIP

Lionnet and Singer Genome Biology 2010,11:129 /2010/11/8/129 RESEARCH HIGHLIGHT Transcription, one allele at a time Timothée Lionnetand Robert H Singer* bacteriophage capsid protein (MCP). Coexpression of the Abstract MBS-gene construct with one in which MCP is fused to a ?uorescent protein gives you an endogenous reporter system that allows the detection of single molecules of mRNA (Figure 1a). A recent study presents a technique allowing one to image transcription from a single gene copy in live cells, and highlights the dynamic nature of transcriptional regulation. The MS2 RNA reporter system combined with site-specic recombination Transcription of a gene into its messenger RNA occurs Using the MS2 system, details of mRNA transcription, through a series of de?ned steps, from transcription factor binding at the promoter to pre-initiation complex di?usion and nuclear export have been revealed in many di?erent organisms, from bacteria to fruit ?ies to formation, entry into elongation and ?nally termination mammalian cells [3]. Up to now, however, genomic [1]. Most of the players in these processes, such as the integration of MS2-tagged gene constructs using tradi- general transcription factors associated with the RNA tional techniques (such as plasmid integration or viral polymerase, elongation factors, and termination factors, infection) has resulted in the integration at a random are now well known. However, transcription is inherently a highly dynamic process [2], which sets obvious experi- genomic location of an array containing multiple copies of the gene of interest [6]. Although these arrays confer mental challenges to most biochemical methods. In this high ampli?cation of ?uorescent signal, they come with context, imaging techniques have emerged as important drawbacks. First, the real-time signa