Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates 英文参考文献.docVIP

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Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates 英文参考文献.doc

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Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates 英文参考文献

/2001/2/2/research/0006.1 Research Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogeneSHMT-ps1 is unique to the order Primates Eric J Devor Address: Molecular Genetics and Bioinformatics, Integrated DNA Technologies, Inc., 1710 Commercial Park, Coralville, IA 52241, USA. E-mail: rdevor@ Published: 29 January 2001 Received: 22 November 2000 Revised: 20 December 2000 Accepted: 2 January 2001 GenomeBiology 2001, 2(2):research0006.1–0006.5 The electronic version of this article is the complete one and can be found online at /2001/2/2/research/0006 ? BioMed Central Ltd (Print ISSN 1465-6906; Online ISSN 1465-6914) Abstract Background: The serine hydroxymethyltransferase processed pseudogene SHMT-ps1 has been suggested to be unique to the order Primates because of the failure to amplify this sequence by PCR from genomic DNAs of any non-primate mammal species. Here, ‘molecular beacon’ probes specific toSHMT-ps1 were used in an attempt to verify this suggestion. Results: In a search for SHMT-ps1-specific sequences using molecular beacons across a range of mammalian species,SHMT-ps1 was only found in primates. The molecular beacon assays also showed thatSHMT-ps1 is present in both Old World and New World species but not among prosimians. Conclusions: These results suggest that SHMT-ps1 originated close to the origin of the Anthropoidea, some 40 to 50 million years ago. Background PCR-based analyses of evolutionary conservation of the SHMT-ps1 nucleotide sequence suggest that the reverse tran- scription event giving rise to SHMT-ps1 occurred after the divergence of the order Primates from the rest of the Mam- malia, thus making this locus a genetic marker unique to the primates [7]. Here, this suggestion is verified using two end- point molecular beacon assays. In addition, results presented here further refine the time frame in which the reverse tran- scription and re