第十二章 细胞的衰老.pptVIP

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * While the annexin-V binding assay is fairly robust when used in suspension growing cells, its use can be a little tricky when assessing apoptosis in adherent cells. The most common methods of cell detachment use trypsinisation and/or EDTA, both of which can seriously affect the assay. Trypsinisation can affect both membrane integrity and PS localisation and EDTA will sequester Ca2+, which is essential for the binding of annexin-V. It is therefore important to include the appropriate controls and optimise the assay for each cel