绵羊微卫星标记与体重的相.docVIP

绵羊微卫星标记与体重的相关分析 孙业良1，刘国庆1，2，王 刚3，任航行2，代 蓉2，刘守仁2，谢 庄1 (1南京农业大学动物科技学院，南京210095；2新疆农垦科学院畜牧研究所，石河子 832000；3西部牧业集团，石河子 832000） 摘要：【目的】研究微卫星标记与肉用品系中国美利奴羊体重的相关关系，为采用标记辅助选择的方法提高选育效果，和进一步加快育种进展提供一定参考依据。【方法】根据绵羊的遗传图谱及相关报道选择了位于4号和6号染色体上连锁的10个微卫星基因座对162只肉用品系中国美利奴羊群体进行群体遗传学检测；计算其有效等位基因数、杂合度、多态信息含量，并进行微卫星标记与体重的相关性分析。【结果】微卫星不同基因型在体重上的多重比较和微卫星标记对体重相关效应的F检验结果表明：紧密连锁的基因座OARHH35 和BMS648与体重间存在显著的差异（P0.05），有一个影响体重的QTL；染色体进行同源比较发现表明：在这两个座位之间可能存在ob基因。【结论】6个微卫星基因座的基因型[BM9058（131/149）、BM4621（149/181）、BM4311（119/119）、OARJMP8（131/145）、OARHH35（123/155）和BMS648（176/208）]对体重存在正相关性。 关键词：肉用绵羊；微卫星；体重 Correlation Analysis Between Microsatellite Marks and Body Weight in Meat Sheep SUN Ye-liang1, LIU Guo-qing1,2, WANG Gang3, REN Hang-xing2, DAI Rong2, LIU Shou-ren2, XIE Zhuang1 ( 1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095; 2Research Institute of Farming Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000; 3Western Animal Husbandry Group, Shihezi 832000 ) Abstract：bjective】Researching the correlation between microsatellite marks and body weight in Chinese Merino; in order to use of marker-assisted selection methods to improve breeding results, and to provide a basis for further progress to accelerate breeding.【Method】The amplified fragment length polymorphism of ten linkage microsatellite loci in four and six chromosome according to the sheep linkage map and reports were detected in 162 meat sheep of Chinese Merino. Effective number of alleles, heterozygosity, polymorphism information content, and correlation analysis between microsatellite marks and body weight as well were calculated for ten microsatellite loci in Chinese Merino. 【Result】By comparing genotype weight in ten microsatellite loci and administering an F test for the relativity of microsatellite markers with weight traits, the results showed that two close linkage OARHH35 and BMS648 had a significant d