不同激活方法对猪ICSI卵早期发育的影响.PDFVIP

安徽农业科学,Journal of Anhui Agri . Sci .2009 ,37( 7) :2983 - 2985 ,3000 责任编辑 李玮 责任校对 卢瑶 不同激活方法对猪I CSI 卵早期发育的影响 1,2 1,2 1 ,2 2 1 ,2 2 3 3 3 张玲 , 冯宝刚 , 许惠艳 , 何若钢 , 卢晟盛 , 房慧伶 , 潘天彪 , 蓝海恩, 黄敏瑞 , 1 ,2 * 卢克焕 ( 1. 广西大学广西亚热带生物资源保护利用重点实验室, 广西南宁530005 ;2 . 广西大学动物科学技术学院, 广西南宁530005 ;3 . 广西畜牧研究所, 广西南宁530001) 摘要 [ 目的] 探讨不 同激活方法对猪单精子显微 受精(ICSI) 胚发育的影 响。[ 方法] 冷冻精子解 冻洗涤后 直接应 用于ICSI ,I CSI 后采 用 不 同激活方法激活卵母细胞, 检测其原核形成情况。[ 结果] 结果表 明, 单纯电脉冲激活以及电脉冲与6- DMAP 联合激活其受精率分别达 53 .3 % 和60 .4 % , 对ICSI 卵雌雄原核形成的影响差异不显著( P 0 .05) ; I CSI 后采 用不 同激活方法激活卵母细胞, 检测猪ICSI 卵胚胎早 期发育情况, 单纯电脉冲激活囊胚率达13 .0 % , 显著高于对照( P 0 .05) 。[ 结论] 单纯电脉冲激活能促进猪ICSI 胚胎的早期发育。 关键词 猪; 卵母细胞;ICSI ; 电激活 中图分类号 S828 文献标识码 A 文章编号 0517 - 6611(2009) 07 - 02983 - 03 Effect of Various Methods of Oocyte Activation on Development of Porcine I CSI Oocytes ZHANG Ling et al ( Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization , Guangxi University , Nanning , Guangxi 530005) Abstract [ Obj ective] The purpose of this study was to deter mi ne the effect of various methods of oocyte activation on development of porcine embryos de- rived fromintracytoplasmic sper minj ection (ICSI) .[ Method] Oocytes inj ected with frozen-thawed sper mwere activated by various methods , and the effect on the pronucleus for mation and blastocyst for mation rate were examined . [ Result] The results indicated that the fertilization rates of 53 .3 % and 60 .4 % were achieved respectively when electric activation and electricity combined with 6- di methylaminopurine ( 6- DMAP) were used . The best rate of blastocyst for mation was achieved ( 13 .0 %) among the three groups of ICSI oocytes when electric