A new immunoprecipitation-real time quantitative PCR assay for anti-ThTo and anti-U3RNP antibody detection in systemic sclerosis.docVIP

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A new immunoprecipitation-real time quantitative PCR assay for anti-ThTo and anti-U3RNP antibody detection in systemic sclerosis.doc

A new immunoprecipitation-real time quantitative PCR assay for anti-ThTo and anti-U3RNP antibody detection in systemic sclerosis

Ceribellietal.ArthritisResearchTherapy2012,14:R128 /content/14/3/R128 RESEARCH ARTICLE OpenAccess Anewimmunoprecipitation-realtime quantitativePCRassayforanti-Th/Toandanti- U3RNPantibodydetectioninsystemicsclerosis AngelaCeribelli1,MinoruSatoh2andEdwardKLChan1* Abstract Introduction:Classicanti-nucleolarantibodiesanti-Th/ToandU3ribonucleoprotein(-U3RNP)canhelpinthe diagnosis,predictionoforganinvolvementandprognosisinsystemicsclerosis(SSc);however,novalidated commercialassayisavailable.WeaimedatestablishinganovelquantitativerealtimePCR(qPCR)methodto detecttheseantibodies. Methods:Standardimm

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A new immunoprecipitation-real time quantitative PCR assay for anti-ThTo and anti-U3RNP antibody detection in systemic sclerosis.docVIP