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A rapid and easy method for the DNA extraction from Cryptococcus neoformans
Mseddietal.BiologicalProceduresOnline2011,13:5
/content/13/1/5
Biological Procedures
Online
RESEARCH
OpenAccess
ArapidandeasymethodfortheDNAextraction
fromCryptococcusneoformans
FatmaMseddi,MohammedAliJarboui,AmiraSellami,HayetSellamiandAliAyadi*
Abstract
DNAisolationfromC.neoformansisdifficultduetoathickandresistantcapsule.Wehaveoptimizedanewand
rapidDNAisolationmethodforCryptococcususingashortureatreatmentfollowedbyarapidmethodusinga
chelexresinsuspension.Thisprocedureissimplerthanpreviouslyreportedmethods.
Introduction
Materialsandmethods
NucleicaciddetectionmethodssuchasPCRhavebecome
Str
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