A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes.docVIP

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A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes.doc

A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes

Wangetal.AnnalsofClinicalMicrobiologyandAntimicrobials2012,11:9 /content/11/1/9 RESEARCH OpenAccess Arapidlow-costreal-timePCRforthedetection ofklebsiellapneumoniacarbapenemasegenes LijunWang,HaitongGuandXinxinLu* Abstract Background:Klebsiellapneumoniacarbapenemases(KPCs)areabletohydrolyzethecarbapenems,whichcause manybacteriaresistancetomultipleclassesofantibiotics,sotherapiddisseminationofKPCsisworrisome. LaboratoryidentificationofKPCs-harboringclinicalisolateswouldbeakeytolimitthespreadofthebacteria.This studywouldevaluatearapidlow-costreal-timePCRassaytodetectKPCs. Methods:Real-timePCRa

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