A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes.docVIP
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A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes
Wangetal.AnnalsofClinicalMicrobiologyandAntimicrobials2012,11:9
/content/11/1/9
RESEARCH
OpenAccess
Arapidlow-costreal-timePCRforthedetection
ofklebsiellapneumoniacarbapenemasegenes
LijunWang,HaitongGuandXinxinLu*
Abstract
Background:Klebsiellapneumoniacarbapenemases(KPCs)areabletohydrolyzethecarbapenems,whichcause
manybacteriaresistancetomultipleclassesofantibiotics,sotherapiddisseminationofKPCsisworrisome.
LaboratoryidentificationofKPCs-harboringclinicalisolateswouldbeakeytolimitthespreadofthebacteria.This
studywouldevaluatearapidlow-costreal-timePCRassaytodetectKPCs.
Methods:Real-timePCRa
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