A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital.docVIP
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A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital
Hardegenetal.AnnalsofClinicalMicrobiologyandAntimicrobials2010,9:5
/content/9/1/5
RESEARCH
OpenAccess
AsetofnovelmultiplexTaqmanreal-timePCRs
forthedetectionofdiarrhoeagenicEscherichia
colianditsuseindeterminingtheprevalenceof
EPECandEAECinauniversityhospital
ChristophHardegen1?,SabineMessler1?,BirgitHenrich1,KlausPfeffer1,JensWürthner2,ColinRMacKenzie1*
Abstract
Background:AccuratemeasurementoftheincidenceofdiarrhoeagenicE.coliinpatientswithdiarrhoeais
hinderedbythecurrentmethodsofdetectionandvariesfromcountrytocountry.Inordertoimprovethe
diagnosisofdiarrhoeagenicE.coli(DEC),wedevelopedaset
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