A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital.docVIP

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A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital.doc

A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital

Hardegenetal.AnnalsofClinicalMicrobiologyandAntimicrobials2010,9:5 /content/9/1/5 RESEARCH OpenAccess AsetofnovelmultiplexTaqmanreal-timePCRs forthedetectionofdiarrhoeagenicEscherichia colianditsuseindeterminingtheprevalenceof EPECandEAECinauniversityhospital ChristophHardegen1?,SabineMessler1?,BirgitHenrich1,KlausPfeffer1,JensWürthner2,ColinRMacKenzie1* Abstract Background:AccuratemeasurementoftheincidenceofdiarrhoeagenicE.coliinpatientswithdiarrhoeais hinderedbythecurrentmethodsofdetectionandvariesfromcountrytocountry.Inordertoimprovethe diagnosisofdiarrhoeagenicE.coli(DEC),wedevelopedaset

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