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5 7 Vol. 5 No. 7 2014 7 Journal of Food Safety and Quality Jul. , 2014 α- 1 1 2 2* 陈 启 , 赖世云 , 张京顺 , 任一平 (1. , 310057; 2. , 310051) 摘 要: 目的 α-方法 α- , ; 结果 , α-8.0 mg/100 g, 5.22%, 97.21%~102.45%结论 α-, 447 , α- , 关键词: ; ; α-; Quantification of α-lactalbumin in human milk based on ultra-performance liquid chromatography coupled with tandem mass spectrometry 1 1 2 2* CHEN Qi , LAI Shi-Yun , ZHANG Jing-Shun , REN Yi-Ping (1. Beingmate Baby Child Food Co., Ltd., Hangzhou 310057, China; 2. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China) ABSTRACT: Objective To establish a quantitative detection of α-lactalbumin in human milk with peptide standard system. Methods The signature peptide for α-lactalbumin was searched and selected from the pro- tein digestion products. The signature peptide, isotope-labelled signature peptide and internal standard peptide were also synthesized and used for establishing the quantification method. Results The limit of quantification for α-lactalbumin was 8.0 mg/100 g. Reproducibility were below 5.22% and spiked recoveries were between 97.21%~102.45%. Conclusion The validation showed that the