原创力文档Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures.docVIP
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Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures
Ribasetal.BMCPlantBiology2011,11:92
/1471-2229/11/92
RESEARCH ARTICLE
OpenAccess
Agrobacterium-mediatedgenetictransformation
ofCoffeaarabica(L.)isgreatlyenhancedby
usingestablishedembryogeniccalluscultures
AlessandraFRibas1,EvelineDechamp1,AnthonyChampion2,Beno?tBertrand1,Marie-ChristineCombes2,
Jean-LucVerdeil3,FabienneLapeyre3,PhilippeLashermes2andHervéEtienne1*
Abstract
Background:Followinggenomesequencingofcropplants,oneofthemainchallengestodayisdeterminingthe
functionofallthepredictedgenes.Whengenevalidationapproachesareusedforwoodyspecies,themain
obstacleisthelowrecoveryrateoftransgen
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