应用酶联免疫法检测鱼肉、蜂蜜中氯霉素的残留量.pdf

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.12 应用酶联免疫法检测鱼肉、蜂蜜中氯霉素的残留量 1,2 1 1 1,2 彭运 ，齐维，唐海波，刘晓云 （1.广州万孚生物技术有限公司，广东广州 510530 ） （2.华南理工大学轻工与食品学院，广东广州 510641 ） 摘要：检测鱼、蜂蜜中氯霉素残留，以评价试剂盒性能为目的。用间接竞争酶联免疫分析法（ELISA法）对鱼肉、蜂蜜 2 中的氯霉素残留量进行了测定。结果表明，标准曲线的R 为0.9943 ，线性方程y=-0.3668x+0.4353 ，IC50为0.71 μg/L，线性范围为0.05~4.05 ng/mL 。鱼肉和蜂蜜中的检测限分别为0.126 μg/kg和0.081 μg/kg。鱼肉和蜂蜜的添加回收率分别大于88.1%和85.5%，批内变异系数分别小于15.8%、13.6%，批间变异系数小于15.5%、1 2.3% 。与氯霉素琥珀酸钠的交叉反应率为152.63%，与其他药物的交叉反应率均小于0.01%。该法测定氯霉素灵敏准确，重复 性好，特异性高，适合用于检测鱼肉、蜂蜜中的氯霉素残留。 关键词：氯霉素；酶联免疫法；鱼肉；蜂蜜 文章篇号：1673-9078(2010)12-1415-1417 Detection the Residues of Chloramphenicol in Fish Meat and Honey Using Enzyme-linked Immunosorbent Assay 1,2 1 1 1,2 PENG Yun-ping , QI Wei , TANG Hai-bo , LIU Xiao-yun (1.Guangzhou Wondfo Biotech Co., Ltd, Guangzhou 510530, China) (2.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China) Abstract: A indirect competitive enzyme-linked immunosorbent assay (ELISA) was used to detect the residues of chloramphenicol in fish meat and honey. The results showed that R2 of the standard curve was 0.9943, and the linear equation was: y=- 0.3668x+0.4353, IC50=0.71 μg/L. Quantitation of chloramphenicol was linear from 0.05 ng/ml to 4.05 ng/ml. The limits of detection were 0.126 μg/kg in fish meat and 0.081 μg/kg in honey, respectively. The recovery rates in fish meat and honey were more than 88.1% and 85.5%, respectively, inter-assay coefficient v