原创力文档BSmooth from whole genome bisulfite sequencing reads to differentially methylated regions.docVIP
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BSmooth from whole genome bisulfite sequencing reads to differentially methylated regions
Hansenetal.GenomeBiology2012,13:R83
/2012/13/10/R83
METHOD
OpenAccess
BSmooth:fromwholegenomebisulfite
sequencingreadstodifferentiallymethylated
regions
KasperDHansen1*?,BenjaminLangmead1,2*?andRafaelAIrizarry1,2*
Abstract
DNAmethylationisanimportantepigeneticmodificationinvolvedingeneregulation,whichcannowbe
measuredusingwhole-genomebisulfitesequencing.However,cost,complexityofthedata,andlackof
comprehensiveanalyticaltoolsaremajorchallengesthatkeepthistechnologyfrombecomingwidelyapplied.
HerewepresentBSmooth,analignment,qualitycontrolandanalysispipelinethatprovidesaccurateandprecise
results
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